LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis
(2018) In Nature 557(7703). p.112-117- Abstract
The linear ubiquitin chain assembly complex (LUBAC) is required for optimal gene activation and prevention of cell death upon activation of immune receptors, including TNFR1 1 . Deficiency in the LUBAC components SHARPIN or HOIP in mice results in severe inflammation in adulthood or embryonic lethality, respectively, owing to deregulation of TNFR1-mediated cell death 2-8 . In humans, deficiency in the third LUBAC component HOIL-1 causes autoimmunity and inflammatory disease, similar to HOIP deficiency, whereas HOIL-1 deficiency in mice was reported to cause no overt phenotype 9-11 . Here we show, by creating HOIL-1-deficient mice, that HOIL-1 is as essential for LUBAC function as HOIP, albeit for... (More)
The linear ubiquitin chain assembly complex (LUBAC) is required for optimal gene activation and prevention of cell death upon activation of immune receptors, including TNFR1 1 . Deficiency in the LUBAC components SHARPIN or HOIP in mice results in severe inflammation in adulthood or embryonic lethality, respectively, owing to deregulation of TNFR1-mediated cell death 2-8 . In humans, deficiency in the third LUBAC component HOIL-1 causes autoimmunity and inflammatory disease, similar to HOIP deficiency, whereas HOIL-1 deficiency in mice was reported to cause no overt phenotype 9-11 . Here we show, by creating HOIL-1-deficient mice, that HOIL-1 is as essential for LUBAC function as HOIP, albeit for different reasons: whereas HOIP is the catalytically active component of LUBAC, HOIL-1 is required for LUBAC assembly, stability and optimal retention in the TNFR1 signalling complex, thereby preventing aberrant cell death. Both HOIL-1 and HOIP prevent embryonic lethality at mid-gestation by interfering with aberrant TNFR1-mediated endothelial cell death, which only partially depends on RIPK1 kinase activity. Co-deletion of caspase-8 with RIPK3 or MLKL prevents cell death in Hoil-1 -/- (also known as Rbck1 -/-) embryos, yet only the combined loss of caspase-8 with MLKL results in viable HOIL-1-deficient mice. Notably, triple-knockout Ripk3 -/- Casp8 -/- Hoil-1 -/- embryos die at late gestation owing to haematopoietic defects that are rescued by co-deletion of RIPK1 but not MLKL. Collectively, these results demonstrate that both HOIP and HOIL-1 are essential LUBAC components and are required for embryogenesis by preventing aberrant cell death. Furthermore, they reveal that when LUBAC and caspase-8 are absent, RIPK3 prevents RIPK1 from inducing embryonic lethality by causing defects in fetal haematopoiesis.
(Less)
- author
- publishing date
- 2018-05-03
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature
- volume
- 557
- issue
- 7703
- pages
- 6 pages
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85046452035
- pmid:29695863
- ISSN
- 0028-0836
- DOI
- 10.1038/s41586-018-0064-8
- language
- English
- LU publication?
- no
- id
- 0f2e8d68-ef68-4cf7-9e87-2358987e1292
- date added to LUP
- 2018-08-31 12:20:58
- date last changed
- 2024-04-15 11:53:54
@article{0f2e8d68-ef68-4cf7-9e87-2358987e1292,
abstract = {{<p>The linear ubiquitin chain assembly complex (LUBAC) is required for optimal gene activation and prevention of cell death upon activation of immune receptors, including TNFR1 <sup>1</sup> . Deficiency in the LUBAC components SHARPIN or HOIP in mice results in severe inflammation in adulthood or embryonic lethality, respectively, owing to deregulation of TNFR1-mediated cell death <sup>2-8</sup> . In humans, deficiency in the third LUBAC component HOIL-1 causes autoimmunity and inflammatory disease, similar to HOIP deficiency, whereas HOIL-1 deficiency in mice was reported to cause no overt phenotype <sup>9-11</sup> . Here we show, by creating HOIL-1-deficient mice, that HOIL-1 is as essential for LUBAC function as HOIP, albeit for different reasons: whereas HOIP is the catalytically active component of LUBAC, HOIL-1 is required for LUBAC assembly, stability and optimal retention in the TNFR1 signalling complex, thereby preventing aberrant cell death. Both HOIL-1 and HOIP prevent embryonic lethality at mid-gestation by interfering with aberrant TNFR1-mediated endothelial cell death, which only partially depends on RIPK1 kinase activity. Co-deletion of caspase-8 with RIPK3 or MLKL prevents cell death in Hoil-1 <sup>-/-</sup> (also known as Rbck1 <sup>-/-</sup>) embryos, yet only the combined loss of caspase-8 with MLKL results in viable HOIL-1-deficient mice. Notably, triple-knockout Ripk3 <sup>-/-</sup> Casp8 <sup>-/-</sup> Hoil-1 <sup>-/-</sup> embryos die at late gestation owing to haematopoietic defects that are rescued by co-deletion of RIPK1 but not MLKL. Collectively, these results demonstrate that both HOIP and HOIL-1 are essential LUBAC components and are required for embryogenesis by preventing aberrant cell death. Furthermore, they reveal that when LUBAC and caspase-8 are absent, RIPK3 prevents RIPK1 from inducing embryonic lethality by causing defects in fetal haematopoiesis.</p>}},
author = {{Peltzer, Nieves and Darding, Maurice and Montinaro, Antonella and Draber, Peter and Draberova, Helena and Kupka, Sebastian and Rieser, Eva and Fisher, Amanda and Hutchinson, Ciaran and Taraborrelli, Lucia and Hartwig, Torsten and Lafont, Elodie and Haas, Tobias L. and Shimizu, Yutaka and Böiers, Charlotta and Sarr, Aida and Rickard, James and Alvarez-Diaz, Silvia and Ashworth, Michael T. and Beal, Allison and Enver, Tariq and Bertin, John and Kaiser, William and Strasser, Andreas and Silke, John and Bouillet, Philippe and Walczak, Henning}},
issn = {{0028-0836}},
language = {{eng}},
month = {{05}},
number = {{7703}},
pages = {{112--117}},
publisher = {{Nature Publishing Group}},
series = {{Nature}},
title = {{LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis}},
url = {{http://dx.doi.org/10.1038/s41586-018-0064-8}},
doi = {{10.1038/s41586-018-0064-8}},
volume = {{557}},
year = {{2018}},
}