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Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays

Ellmark, Peter LU ; Högerkorp, Carl-Magnus LU ; Ek, Sara LU ; Beelov, Larissa ; Berglund, Mattias ; Rosenquist, Richard ; Christopherson, Richard and Borrebaeck, Carl LU (2008) In Cancer Letters 265. p.98-106
Abstract
Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cancer Letters
volume
265
pages
98 - 106
publisher
Elsevier
external identifiers
  • pmid:18353541
  • wos:000256730200010
  • scopus:43449096332
  • pmid:18353541
ISSN
1872-7980
DOI
10.1016/j.canlet.2008.02.006
language
English
LU publication?
yes
id
173039bc-1900-4f72-b0f0-acff3001dfa1 (old id 1028778)
date added to LUP
2016-04-01 13:26:11
date last changed
2022-01-27 19:14:07
@article{173039bc-1900-4f72-b0f0-acff3001dfa1,
  abstract     = {{Antibody microarrays enable extensive protein expression profiling, and provide a valuable complement to DNA microarray-based gene expression profiling. In this study, we used DotScan™ antibody microarrays that contain antibodies against 82 different cell surface antigens, to determine phenotypic protein expression profiles for human B cell sub-populations. We then demonstrated that the B cell protein profile can be used to delineate the relationship between normal B cells and malignant counterparts. Principle component analysis showed that the lymphomas did not cluster with the normal memory B cells or germinal centre B cells, but they did cluster with germinal centre founder cells and naïve B cells.}},
  author       = {{Ellmark, Peter and Högerkorp, Carl-Magnus and Ek, Sara and Beelov, Larissa and Berglund, Mattias and Rosenquist, Richard and Christopherson, Richard and Borrebaeck, Carl}},
  issn         = {{1872-7980}},
  language     = {{eng}},
  pages        = {{98--106}},
  publisher    = {{Elsevier}},
  series       = {{Cancer Letters}},
  title        = {{Phenotypic protein profiling of different B cell sub-populations using antibody CD-microarrays}},
  url          = {{http://dx.doi.org/10.1016/j.canlet.2008.02.006}},
  doi          = {{10.1016/j.canlet.2008.02.006}},
  volume       = {{265}},
  year         = {{2008}},
}