Monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization.
(2008) In Analytical and Bioanalytical Chemistry 390(3). p.907-912- Abstract
- A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material. In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the... (More)
- A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material. In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the matrix allowed for the development of a generic, high-performance online protein digestion system utilizing the wall-bound proteases. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1035491
- author
- Hedström, Martin LU ; Plieva, Fatima LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chitosan, Macroporous, Cryogel, Scaffold, Staphylococcal enterotoxin B
- in
- Analytical and Bioanalytical Chemistry
- volume
- 390
- issue
- 3
- pages
- 907 - 912
- publisher
- Springer
- external identifiers
-
- pmid:18066683
- wos:000252417800015
- scopus:38348999498
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-007-1745-6
- language
- English
- LU publication?
- yes
- id
- adda712b-23e1-4663-b094-8f37581d1a28 (old id 1035491)
- date added to LUP
- 2016-04-01 12:06:53
- date last changed
- 2022-01-26 22:57:31
@article{adda712b-23e1-4663-b094-8f37581d1a28,
abstract = {{A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material. In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the matrix allowed for the development of a generic, high-performance online protein digestion system utilizing the wall-bound proteases.}},
author = {{Hedström, Martin and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo}},
issn = {{1618-2642}},
keywords = {{Chitosan; Macroporous; Cryogel; Scaffold; Staphylococcal enterotoxin B}},
language = {{eng}},
number = {{3}},
pages = {{907--912}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization.}},
url = {{http://dx.doi.org/10.1007/s00216-007-1745-6}},
doi = {{10.1007/s00216-007-1745-6}},
volume = {{390}},
year = {{2008}},
}