Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells.
(2002) In Neuron 33(2). p.287-299- Abstract
- Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion... (More)
- Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion pore is negligible and only proceeds after complete fusion, and (3) sluggish peptidergic transmission reflects the time course of vesicle emptying. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106536
- author
- Barg, Sebastian LU ; Olofsson, Charlotta LU ; Schriever-Abeln, Jenny ; Wendt, Anna LU ; Gebre-Medhin, Samuel LU ; Renström, Erik LU and Rorsman, Patrik LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Peptides/*metabolism, PC12 Cells, Neurosecretory Systems/cytology/*metabolism, Membrane Fusion/*physiology, Luminescent Proteins, Kinetics, Indicators and Reagents, Hydrogen-Ion Concentration, Exocytosis/*physiology, Fluorescence, Rats, Secretory Vesicles/*metabolism, Support, Non-U.S. Gov't, Electric Stimulation, Cell Line, Animal
- in
- Neuron
- volume
- 33
- issue
- 2
- pages
- 287 - 299
- publisher
- Cell Press
- external identifiers
-
- wos:000173387700015
- pmid:11804575
- scopus:0037122871
- ISSN
- 0896-6273
- DOI
- 10.1016/S0896-6273(02)00563-9
- language
- English
- LU publication?
- yes
- id
- a6b3a7f9-3ba8-45cb-9600-ae4a2b497f1f (old id 106536)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11804575&dopt=Abstract
- date added to LUP
- 2016-04-01 12:31:53
- date last changed
- 2022-02-26 08:22:15
@article{a6b3a7f9-3ba8-45cb-9600-ae4a2b497f1f,
abstract = {{Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion pore is negligible and only proceeds after complete fusion, and (3) sluggish peptidergic transmission reflects the time course of vesicle emptying.}},
author = {{Barg, Sebastian and Olofsson, Charlotta and Schriever-Abeln, Jenny and Wendt, Anna and Gebre-Medhin, Samuel and Renström, Erik and Rorsman, Patrik}},
issn = {{0896-6273}},
keywords = {{Peptides/*metabolism; PC12 Cells; Neurosecretory Systems/cytology/*metabolism; Membrane Fusion/*physiology; Luminescent Proteins; Kinetics; Indicators and Reagents; Hydrogen-Ion Concentration; Exocytosis/*physiology; Fluorescence; Rats; Secretory Vesicles/*metabolism; Support; Non-U.S. Gov't; Electric Stimulation; Cell Line; Animal}},
language = {{eng}},
number = {{2}},
pages = {{287--299}},
publisher = {{Cell Press}},
series = {{Neuron}},
title = {{Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells.}},
url = {{http://dx.doi.org/10.1016/S0896-6273(02)00563-9}},
doi = {{10.1016/S0896-6273(02)00563-9}},
volume = {{33}},
year = {{2002}},
}