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Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.

Bal, Karol LU ; Larsson, Lennart LU ; Mielniczuk, Eugenia and Mielniczuk, Zbigniew (2002) In Journal of Microbiological Methods 48(2-3). p.267-270
Abstract
The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muramic Acids : chemistry, Trimethylsilyl Compounds : chemistry, Spectrum Analysis Mass, Peptidoglycan : analysis, Bacteria : chemistry
in
Journal of Microbiological Methods
volume
48
issue
2-3
pages
267 - 270
publisher
Elsevier
external identifiers
  • wos:000173483900016
  • pmid:11777574
  • scopus:0036147685
ISSN
1872-8359
DOI
10.1016/S0167-7012(01)00328-1
language
English
LU publication?
yes
id
4dd67462-04e3-4d4d-8bdf-38ac8102c4a5 (old id 106862)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11777574&dopt=Abstract
date added to LUP
2016-04-01 12:20:34
date last changed
2022-01-27 02:18:50
@article{4dd67462-04e3-4d4d-8bdf-38ac8102c4a5,
  abstract     = {{The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.}},
  author       = {{Bal, Karol and Larsson, Lennart and Mielniczuk, Eugenia and Mielniczuk, Zbigniew}},
  issn         = {{1872-8359}},
  keywords     = {{Muramic Acids : chemistry; Trimethylsilyl Compounds : chemistry; Spectrum Analysis Mass; Peptidoglycan : analysis; Bacteria : chemistry}},
  language     = {{eng}},
  number       = {{2-3}},
  pages        = {{267--270}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Microbiological Methods}},
  title        = {{Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.}},
  url          = {{http://dx.doi.org/10.1016/S0167-7012(01)00328-1}},
  doi          = {{10.1016/S0167-7012(01)00328-1}},
  volume       = {{48}},
  year         = {{2002}},
}