Eosinophil infiltration and activation at the gastric ulcer margin in rats.
(2002) In European Journal of Gastroenterology and Hepathology 14(5). p.503-511- Abstract
- BACKGROUND : Recruitment and activation of eosinophils have been studied intensely in asthma and other allergic diseases. Less is known about the infiltration and behaviour of eosinophils during gastric ulcer healing. AIM : To examine the tissue infiltration and activation of eosinophils in the ulcer margin at different time points after ulcer induction (days 1-15). METHODS : Eosinophil peroxidase (EPO) staining and transmission electron microscopy (TEM) were used to observe eosinophil infiltration and activation in rats with acetic-acid-induced ulcer in the oxyntic mucosa. The distribution of macrophages was evaluated by immunocytochemistry using the macrophage-specific antibodies ED1 and ED2. RESULTS : There was a prominent increase in... (More)
- BACKGROUND : Recruitment and activation of eosinophils have been studied intensely in asthma and other allergic diseases. Less is known about the infiltration and behaviour of eosinophils during gastric ulcer healing. AIM : To examine the tissue infiltration and activation of eosinophils in the ulcer margin at different time points after ulcer induction (days 1-15). METHODS : Eosinophil peroxidase (EPO) staining and transmission electron microscopy (TEM) were used to observe eosinophil infiltration and activation in rats with acetic-acid-induced ulcer in the oxyntic mucosa. The distribution of macrophages was evaluated by immunocytochemistry using the macrophage-specific antibodies ED1 and ED2. RESULTS : There was a prominent increase in eosinophils around the ulcer margin at day 1 after ulcer induction, which peaked at day 5. TEM revealed characteristic signs of eosinophil activation, including cytolysis and piecemeal degranulation. Eosinophil cytolysis was the major form of activation, seen most frequently at day 5. A few scattered apoptotic eosinophils could also be observed. In normal controls and sham-operated rats, activated eosinophils were detected rarely. The distribution pattern of infiltrated eosinophils closely resembled that of macrophages at the ulcer margin. However, in the central part of the granulation tissue (at day 5) only macrophages could be found. CONCLUSIONS : There is marked infiltration and signs of activation of eosinophils together with macrophages at the margin of newly formed ulcers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107952
- author
- Mei, Qi ; Korsgren, Magnus LU ; Erjefält, Jonas LU and Sundler, Frank LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Female, Gastric Mucosa : ultrastructure, Macrophage Activation : physiology, Microscopy, Electron, Rats, Sprague-Dawley, Stomach Ulcer : immunology, Stomach Ulcer : physiopathology, Support, Non-U.S. Gov't, Animal, Eosinophils : immunology, Eosinophils : physiology
- in
- European Journal of Gastroenterology and Hepathology
- volume
- 14
- issue
- 5
- pages
- 503 - 511
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:11984148
- wos:000175709600007
- scopus:0036097781
- ISSN
- 1473-5687
- language
- English
- LU publication?
- yes
- id
- 844da6b1-ef95-4d85-85e7-ccb2c6b3b448 (old id 107952)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11984148&dopt=Abstract
- date added to LUP
- 2016-04-01 11:46:42
- date last changed
- 2022-01-26 18:07:50
@article{844da6b1-ef95-4d85-85e7-ccb2c6b3b448,
abstract = {{BACKGROUND : Recruitment and activation of eosinophils have been studied intensely in asthma and other allergic diseases. Less is known about the infiltration and behaviour of eosinophils during gastric ulcer healing. AIM : To examine the tissue infiltration and activation of eosinophils in the ulcer margin at different time points after ulcer induction (days 1-15). METHODS : Eosinophil peroxidase (EPO) staining and transmission electron microscopy (TEM) were used to observe eosinophil infiltration and activation in rats with acetic-acid-induced ulcer in the oxyntic mucosa. The distribution of macrophages was evaluated by immunocytochemistry using the macrophage-specific antibodies ED1 and ED2. RESULTS : There was a prominent increase in eosinophils around the ulcer margin at day 1 after ulcer induction, which peaked at day 5. TEM revealed characteristic signs of eosinophil activation, including cytolysis and piecemeal degranulation. Eosinophil cytolysis was the major form of activation, seen most frequently at day 5. A few scattered apoptotic eosinophils could also be observed. In normal controls and sham-operated rats, activated eosinophils were detected rarely. The distribution pattern of infiltrated eosinophils closely resembled that of macrophages at the ulcer margin. However, in the central part of the granulation tissue (at day 5) only macrophages could be found. CONCLUSIONS : There is marked infiltration and signs of activation of eosinophils together with macrophages at the margin of newly formed ulcers.}},
author = {{Mei, Qi and Korsgren, Magnus and Erjefält, Jonas and Sundler, Frank}},
issn = {{1473-5687}},
keywords = {{Female; Gastric Mucosa : ultrastructure; Macrophage Activation : physiology; Microscopy; Electron; Rats; Sprague-Dawley; Stomach Ulcer : immunology; Stomach Ulcer : physiopathology; Support; Non-U.S. Gov't; Animal; Eosinophils : immunology; Eosinophils : physiology}},
language = {{eng}},
number = {{5}},
pages = {{503--511}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{European Journal of Gastroenterology and Hepathology}},
title = {{Eosinophil infiltration and activation at the gastric ulcer margin in rats.}},
url = {{http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11984148&dopt=Abstract}},
volume = {{14}},
year = {{2002}},
}