Reversal of tolerance induced by transplantation of skin expressing the immunodominant T cell epitope of rat type II collagen entitles development of collagen-induced arthritis but not graft rejection.
(2002) In European Journal of Immunology 32(6). p.1773-1783- Abstract
- Collagen-induced arthritis (CIA) is induced in H-2(q) mice after immunization with rat type II collagen (CII). The immunodominant T cell epitope on heterologous CII has been located to CII256-270. We have previously shown that TSC transgenic mice, which express the heterologous epitope in type I collagen (CI), e.g. in skin, are tolerized against rat CII and resistant to CIA. In this study we transplanted skin from TSC transgenic mice onto non-transgenic CIA-susceptible littermates to investigate whether introduction of this epitope to a naïve immune system would lead to T cell priming and graft rejection or instead to tolerance and arthritis protection. Interestingly, TSC grafts were accepted and not even immunization of recipient mice... (More)
- Collagen-induced arthritis (CIA) is induced in H-2(q) mice after immunization with rat type II collagen (CII). The immunodominant T cell epitope on heterologous CII has been located to CII256-270. We have previously shown that TSC transgenic mice, which express the heterologous epitope in type I collagen (CI), e.g. in skin, are tolerized against rat CII and resistant to CIA. In this study we transplanted skin from TSC transgenic mice onto non-transgenic CIA-susceptible littermates to investigate whether introduction of this epitope to a naïve immune system would lead to T cell priming and graft rejection or instead to tolerance and arthritis protection. Interestingly, TSC grafts were accepted and not even immunization of recipient mice with CII in adjuvant induced graft rejection. Instead, TSC skin recipients displayed a reduced T and B cell response to CII and were also protected from arthritis. However, additional priming could break arthritis protection and was accompanied by an increased T cell response to the grafted epitope. Strikingly, despite the regained T cell response, development of arthritis was not accompanied by graft rejection, showing that these immune-mediated inflammatory responses involve different mechanisms. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109277
- author
- Bäcklund, Johan LU ; Treschow, Alexandra LU ; Firan, Mihail ; Malmström, Vivianne ; Issazadeh, Shohreh LU ; Ward, E Sally and Holmdahl, Rikard LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Epitopes, T-Lymphocyte, Glycosylation, Graft Rejection, Immune Tolerance, Immunodominant Epitopes, Lymphocytes : immunology, Male, Mice, Inbred C3H, Transgenic, Skin Transplantation : immunology, Collagen Type II : immunology, Age Factors, Animal, Arthritis : etiology
- in
- European Journal of Immunology
- volume
- 32
- issue
- 6
- pages
- 1773 - 1783
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000176269000029
- scopus:0036086532
- ISSN
- 1521-4141
- DOI
- 10.1002/1521-4141(200206)32:6<1773::AID-IMMU1773>3.0.CO;2-Z
- language
- English
- LU publication?
- yes
- id
- e38494d6-02d3-4c3a-9386-169c04b10ad8 (old id 109277)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12115661&dopt=Abstract
- date added to LUP
- 2016-04-01 11:45:25
- date last changed
- 2022-01-26 17:45:35
@article{e38494d6-02d3-4c3a-9386-169c04b10ad8,
abstract = {{Collagen-induced arthritis (CIA) is induced in H-2(q) mice after immunization with rat type II collagen (CII). The immunodominant T cell epitope on heterologous CII has been located to CII256-270. We have previously shown that TSC transgenic mice, which express the heterologous epitope in type I collagen (CI), e.g. in skin, are tolerized against rat CII and resistant to CIA. In this study we transplanted skin from TSC transgenic mice onto non-transgenic CIA-susceptible littermates to investigate whether introduction of this epitope to a naïve immune system would lead to T cell priming and graft rejection or instead to tolerance and arthritis protection. Interestingly, TSC grafts were accepted and not even immunization of recipient mice with CII in adjuvant induced graft rejection. Instead, TSC skin recipients displayed a reduced T and B cell response to CII and were also protected from arthritis. However, additional priming could break arthritis protection and was accompanied by an increased T cell response to the grafted epitope. Strikingly, despite the regained T cell response, development of arthritis was not accompanied by graft rejection, showing that these immune-mediated inflammatory responses involve different mechanisms.}},
author = {{Bäcklund, Johan and Treschow, Alexandra and Firan, Mihail and Malmström, Vivianne and Issazadeh, Shohreh and Ward, E Sally and Holmdahl, Rikard}},
issn = {{1521-4141}},
keywords = {{Epitopes; T-Lymphocyte; Glycosylation; Graft Rejection; Immune Tolerance; Immunodominant Epitopes; Lymphocytes : immunology; Male; Mice; Inbred C3H; Transgenic; Skin Transplantation : immunology; Collagen Type II : immunology; Age Factors; Animal; Arthritis : etiology}},
language = {{eng}},
number = {{6}},
pages = {{1773--1783}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{Reversal of tolerance induced by transplantation of skin expressing the immunodominant T cell epitope of rat type II collagen entitles development of collagen-induced arthritis but not graft rejection.}},
url = {{http://dx.doi.org/10.1002/1521-4141(200206)32:6<1773::AID-IMMU1773>3.0.CO;2-Z}},
doi = {{10.1002/1521-4141(200206)32:6<1773::AID-IMMU1773>3.0.CO;2-Z}},
volume = {{32}},
year = {{2002}},
}