A stable, radioactive substrate emulsion for assay of lipoprotein lipase
(1976) In Journal of Lipid Research 17(5). p.536-541- Abstract
- A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and... (More)
- A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1102620
- author
- Nilsson-Ehle, Peter LU and Schotz, Michael C
- publishing date
- 1976
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- adipose lipoprotein lipase tri[3H]oleoylglycerol, triglyceride, postheparin serum
- in
- Journal of Lipid Research
- volume
- 17
- issue
- 5
- pages
- 536 - 541
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:9464
- scopus:0017202148
- ISSN
- 1539-7262
- language
- English
- LU publication?
- no
- id
- e2b82209-7f14-4d86-8583-d1214f49eab2 (old id 1102620)
- alternative location
- http://www.jlr.org/cgi/reprint/17/5/536
- date added to LUP
- 2016-04-01 11:44:46
- date last changed
- 2021-10-10 04:42:26
@article{e2b82209-7f14-4d86-8583-d1214f49eab2,
abstract = {{A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (less than 1 hour), sensitive and reproducible, and suitable for routine use.}},
author = {{Nilsson-Ehle, Peter and Schotz, Michael C}},
issn = {{1539-7262}},
keywords = {{adipose lipoprotein lipase tri[3H]oleoylglycerol; triglyceride; postheparin serum}},
language = {{eng}},
number = {{5}},
pages = {{536--541}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Lipid Research}},
title = {{A stable, radioactive substrate emulsion for assay of lipoprotein lipase}},
url = {{http://www.jlr.org/cgi/reprint/17/5/536}},
volume = {{17}},
year = {{1976}},
}