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Different protein A immunosorbents may have different binding specificity for rat immunoglobulins

Nilsson, Rune LU ; Myhre, E ; Kronvall, G and Sjögren, H O (1983) In Journal of Immunological Methods 62(2). p.241-245
Abstract
Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower uptake of these subclasses. No differences were seen for IgG2a and IgG2c. Protein A on different solid phases cannot be used interchangeably without confirmation of binding specificity.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein A-Sepharose, protein A macrobeads, rat IgG subclasses, immunosorbents
in
Journal of Immunological Methods
volume
62
issue
2
pages
241 - 245
publisher
Elsevier
external identifiers
  • pmid:6350460
  • scopus:0020511158
ISSN
1872-7905
DOI
10.1016/0022-1759(83)90251-X
language
English
LU publication?
yes
id
2ff95b87-5036-49b9-90b7-d6e8f383acef (old id 1103043)
date added to LUP
2016-04-01 16:55:49
date last changed
2021-01-03 10:04:20
@article{2ff95b87-5036-49b9-90b7-d6e8f383acef,
  abstract     = {{Purified polyclonal immunoglobulin preparations representing the 4 rat IgG subclasses were tested for binding to Staphylococcus aureus protein A attached to 3 different solid phases (Staphylococcus aureus Cowan I bacteria, Sepharose CL4B and Sepharose 6MB). Protein A Sepharose CL4B showed higher reactivity with IgG1 and IgG2b than the staphylococci, whereas protein A Sepharose 6MB showed a lower uptake of these subclasses. No differences were seen for IgG2a and IgG2c. Protein A on different solid phases cannot be used interchangeably without confirmation of binding specificity.}},
  author       = {{Nilsson, Rune and Myhre, E and Kronvall, G and Sjögren, H O}},
  issn         = {{1872-7905}},
  keywords     = {{protein A-Sepharose; protein A macrobeads; rat IgG subclasses; immunosorbents}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{241--245}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Immunological Methods}},
  title        = {{Different protein A immunosorbents may have different binding specificity for rat immunoglobulins}},
  url          = {{http://dx.doi.org/10.1016/0022-1759(83)90251-X}},
  doi          = {{10.1016/0022-1759(83)90251-X}},
  volume       = {{62}},
  year         = {{1983}},
}