Extracorporeal immunoadsorption therapy on rats. In vivo depletion of specific antibodies
(1990) In Clinical and Experimental Immunology 82(3). p.440-444- Abstract
- A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to... (More)
- A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to pre-adsorption levels after 4-5 days, or in some cases even exceeded the titres before treatment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105125
- author
- Nilsson, Rune LU ; Lindgren, L and Lilliehorn, P
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- exlracorporeal, immunoadsorplion, antibodies, rats, rebound, overshoot
- in
- Clinical and Experimental Immunology
- volume
- 82
- issue
- 3
- pages
- 440 - 444
- publisher
- British Society for Immunology
- external identifiers
-
- pmid:2265485
- scopus:0025241158
- ISSN
- 0009-9104
- DOI
- 10.1111/j.1365-2249.1990.tb05468.x
- language
- English
- LU publication?
- yes
- id
- 671237db-6578-4fdb-88c2-b1d05a5bc378 (old id 1105125)
- alternative location
- http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1535513
- date added to LUP
- 2016-04-01 11:34:36
- date last changed
- 2021-01-03 05:43:55
@article{671237db-6578-4fdb-88c2-b1d05a5bc378,
abstract = {{A technique for extracorporeal immunoadsorption of circulating specific antibodies from the plasma of rats is described. Catheterization of rats was performed using the carotid and the jugular blood vessels. The rats were treated non-anaesthetized. Blood was pumped continuously through a hollow-fibre plasma filter at a rate of 1.5 ml/min and plasma was separated and passed through an adsorbent column at a flow of 0.2 ml/min. The treated plasma was mixed with the blood cells before being returned to the rat. The column contained the antigen covalently linked to agarose beads. About three plasma volumes were treated during a period of 3 h and 30 min, which resulted in an antibody depletion of about 90-95%. The antibody levels returned to pre-adsorption levels after 4-5 days, or in some cases even exceeded the titres before treatment.}},
author = {{Nilsson, Rune and Lindgren, L and Lilliehorn, P}},
issn = {{0009-9104}},
keywords = {{exlracorporeal; immunoadsorplion; antibodies; rats; rebound; overshoot}},
language = {{eng}},
number = {{3}},
pages = {{440--444}},
publisher = {{British Society for Immunology}},
series = {{Clinical and Experimental Immunology}},
title = {{Extracorporeal immunoadsorption therapy on rats. In vivo depletion of specific antibodies}},
url = {{http://dx.doi.org/10.1111/j.1365-2249.1990.tb05468.x}},
doi = {{10.1111/j.1365-2249.1990.tb05468.x}},
volume = {{82}},
year = {{1990}},
}