Comparison of BrdUrd and [3H]TdR incorporation to estimate cell proliferation, cell loss, and potential doubling time in tumor xenografts
Zätterström, Ulf K ; Johansson, Maria C LU ; Källén, Anders LU ; Baldetorp, Bo LU ; Oredsson, Stina LU ; Wennerberg, Johan LU
and Killander, Dick
LU
(1992)
In Cytometry
13(8).
p.872-879
- Abstract
- In this study, two different methods of estimating cell proliferation were compared: cell loss and potential growth rate of xenografted head and neck cancer grown in nude mice based on the detection of DNA incorporation of bromodeoxyuridine (BrdUrd) in one method, and [3H]thymidine ([3H]TdR) in the other. The 21-d-old xenografts were labelled in vivo, either with BrdUrd or [3H]TdR and excised at intervals during 65.5 h. In tumors containing BrdUrd, the percent labelling was measured in mid-S and mid-G1 phase windows of cytograms from bivariate DNA flow cytometry (FCM). In [3H]TdR-labelled tumors, the percent labelled mitoses (PLM) was determined by light microscopy evaluation of autoradiographs. With a computer program based on a... (More)
- In this study, two different methods of estimating cell proliferation were compared: cell loss and potential growth rate of xenografted head and neck cancer grown in nude mice based on the detection of DNA incorporation of bromodeoxyuridine (BrdUrd) in one method, and [3H]thymidine ([3H]TdR) in the other. The 21-d-old xenografts were labelled in vivo, either with BrdUrd or [3H]TdR and excised at intervals during 65.5 h. In tumors containing BrdUrd, the percent labelling was measured in mid-S and mid-G1 phase windows of cytograms from bivariate DNA flow cytometry (FCM). In [3H]TdR-labelled tumors, the percent labelled mitoses (PLM) was determined by light microscopy evaluation of autoradiographs. With a computer program based on a theoretical model, the percent labelling versus time after injection was used to analyze cell cycle time, cell loss, tumor growth fraction, and potential doubling time. The values calculated from DNA incorporation with BrdUrd agreed well with those obtained from labelling with [3H]TdR, i.e., cell cycle time 2.3 vs. 2.4 d, and growth fraction 67 vs. 70%. The estimated potential doubling time was 3.1 d and cell loss factor 40% by both methods. Flow cytometry analysis of BrdUrd-labelling is considerably faster than the evaluation of [3H]TdR-labelling, and the present results provide further support for the BrdUrd labelling method as a promising alternative to the PLM method in cell cycle studies designed to evaluate the relevance of cell proliferative properties in relation to biological behavior in xenografted head and neck cancer. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1106257
- author
- Zätterström, Ulf K
; Johansson, Maria C
LU
; Källén, Anders
LU
; Baldetorp, Bo
LU
; Oredsson, Stina
LU
; Wennerberg, Johan
LU
and Killander, Dick
LU
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- BrdUrd, xenografts, Cell cycle, head and neck cancer, [3H]thymidine
- in
- Cytometry
- volume
- 13
- issue
- 8
- pages
- 872 - 879
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:1459003
- scopus:0026614388
- ISSN
- 0196-4763
- DOI
- 10.1002/cyto.990130810
- language
- English
- LU publication?
- yes
- id
- cf34c57b-f267-4db9-a198-3d1097cf3621 (old id 1106257)
- date added to LUP
- 2016-04-01 17:04:52
- date last changed
- 2021-01-03 06:09:48
@article{cf34c57b-f267-4db9-a198-3d1097cf3621,
abstract = {{In this study, two different methods of estimating cell proliferation were compared: cell loss and potential growth rate of xenografted head and neck cancer grown in nude mice based on the detection of DNA incorporation of bromodeoxyuridine (BrdUrd) in one method, and [3H]thymidine ([3H]TdR) in the other. The 21-d-old xenografts were labelled in vivo, either with BrdUrd or [3H]TdR and excised at intervals during 65.5 h. In tumors containing BrdUrd, the percent labelling was measured in mid-S and mid-G1 phase windows of cytograms from bivariate DNA flow cytometry (FCM). In [3H]TdR-labelled tumors, the percent labelled mitoses (PLM) was determined by light microscopy evaluation of autoradiographs. With a computer program based on a theoretical model, the percent labelling versus time after injection was used to analyze cell cycle time, cell loss, tumor growth fraction, and potential doubling time. The values calculated from DNA incorporation with BrdUrd agreed well with those obtained from labelling with [3H]TdR, i.e., cell cycle time 2.3 vs. 2.4 d, and growth fraction 67 vs. 70%. The estimated potential doubling time was 3.1 d and cell loss factor 40% by both methods. Flow cytometry analysis of BrdUrd-labelling is considerably faster than the evaluation of [3H]TdR-labelling, and the present results provide further support for the BrdUrd labelling method as a promising alternative to the PLM method in cell cycle studies designed to evaluate the relevance of cell proliferative properties in relation to biological behavior in xenografted head and neck cancer.}},
author = {{Zätterström, Ulf K and Johansson, Maria C and Källén, Anders and Baldetorp, Bo and Oredsson, Stina and Wennerberg, Johan and Killander, Dick}},
issn = {{0196-4763}},
keywords = {{BrdUrd; xenografts; Cell cycle; head and neck cancer; [3H]thymidine}},
language = {{eng}},
number = {{8}},
pages = {{872--879}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Cytometry}},
title = {{Comparison of BrdUrd and [3H]TdR incorporation to estimate cell proliferation, cell loss, and potential doubling time in tumor xenografts}},
url = {{http://dx.doi.org/10.1002/cyto.990130810}},
doi = {{10.1002/cyto.990130810}},
volume = {{13}},
year = {{1992}},
}