Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin

Alling, Christer LU ; Bergman, O ; Larsson, Christer LU and Simonsson, Per LU (1993) In Alcohol and alcoholism. Supplement 2. p.295-299
Abstract
Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA... (More)
Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Alcohol and alcoholism. Supplement
volume
2
pages
295 - 299
publisher
Pergamon Press Ltd.
external identifiers
  • pmid:7748314
  • scopus:0027902710
ISSN
1358-6173
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Tumour Cell Biology (013017530), Clinical Chemistry, Malmö (013016000)
id
4be1e77e-82f3-409d-ab93-9d89a0cf24ce (old id 1106956)
date added to LUP
2016-04-01 16:10:05
date last changed
2021-01-03 07:16:45
@article{4be1e77e-82f3-409d-ab93-9d89a0cf24ce,
  abstract     = {{Human neuroblastoma cells SH-SY5Y and neuroblastoma-glioma cells NG 108-15 have been used as models for the elucidation of the effects of ethanol on receptor-mediated phospholipase C activity, c-fos mRNA expression and protein kinase C activity. Cells were exposed to ethanol (0-200 mM) for varying periods up to seven days. Agonist stimulated events were obtained in NG 108-15 cells with bradykinin and in SH-SY5Y cells with carbachol. Chronic ethanol exposure reduced the agonist-stimulated formation of inositol 1,4,5-trisphosphate in NG 108-15 cells and in SH-SY5Y cells. 100 mM ethanol for seven days increased the membrane bound and cytosolic forms of protein kinase C activity in SH-SY5Y cells. Carbachol (1 mM) induced a maximal c-fos mRNA response after 40 minutes in SH-SY5Y cells, an effect that could be mimicked through protein kinase C stimulation by phorbol esters.}},
  author       = {{Alling, Christer and Bergman, O and Larsson, Christer and Simonsson, Per}},
  issn         = {{1358-6173}},
  language     = {{eng}},
  pages        = {{295--299}},
  publisher    = {{Pergamon Press Ltd.}},
  series       = {{Alcohol and alcoholism. Supplement}},
  title        = {{Evaluation of ethanol effects on PLC signal transduction pathways using cell lines of neuronal origin}},
  volume       = {{2}},
  year         = {{1993}},
}