Functional integration of cortical grafts placed in brain infarcts of rats
(1993) In Annals of Neurology 34(3). p.362-368- Abstract
- Five to 6 days after a right middle cerebral artery occlusion, a cell suspension of fetal neocortex was grafted into the infarcted area of adult spontaneously hypertensive rats. Three to 17 months later, functional integration of the grafts into the afferent somatosensory pathway was tested using the 2-[14C]deoxyglucose method for estimation of glucose utilization. Grafted rats (n = 8) and control rats (n = 5) with no arterial occlusion were stimulated in the left vibrissal region resulting in an increased glucose utilization in the left trigeminal sensory nucleus and the right ventroposterior nucleus of the thalamus, whereas the same regions in a group (n = 5) of nonstimulated grafted rats were not activated. Glucose uptake in the right... (More)
- Five to 6 days after a right middle cerebral artery occlusion, a cell suspension of fetal neocortex was grafted into the infarcted area of adult spontaneously hypertensive rats. Three to 17 months later, functional integration of the grafts into the afferent somatosensory pathway was tested using the 2-[14C]deoxyglucose method for estimation of glucose utilization. Grafted rats (n = 8) and control rats (n = 5) with no arterial occlusion were stimulated in the left vibrissal region resulting in an increased glucose utilization in the left trigeminal sensory nucleus and the right ventroposterior nucleus of the thalamus, whereas the same regions in a group (n = 5) of nonstimulated grafted rats were not activated. Glucose uptake in the right somatosensory cortex of control rats was 96 +/- 5 (mean +/- SEM) mumol/100 gm/min. Neocortical grafts consumed less glucose than cortex in control rats but the vibrissae-stimulated group displayed a 110% higher value than the nonstimulated grafted group (32 +/- 5 vs 15 +/- 2, p < 0.05). We conclude that graft glucose metabolism is increased following stimulation of the host somatosensory pathway, which demonstrates that transplanted neurons can be functionally integrated with neural circuitries of the host after an ischemic insult. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1107336
- author
- Grabowski, Martin LU ; Brundin, Patrik LU and Johansson, Barbro LU
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Annals of Neurology
- volume
- 34
- issue
- 3
- pages
- 362 - 368
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:8363353
- scopus:0027282016
- ISSN
- 1531-8249
- DOI
- 10.1002/ana.410340310
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Neurology, Lund (013027000)
- id
- 6d560c51-d0d3-49ef-a194-434fcf529b83 (old id 1107336)
- date added to LUP
- 2016-04-01 12:32:13
- date last changed
- 2021-05-09 06:01:22
@article{6d560c51-d0d3-49ef-a194-434fcf529b83,
abstract = {{Five to 6 days after a right middle cerebral artery occlusion, a cell suspension of fetal neocortex was grafted into the infarcted area of adult spontaneously hypertensive rats. Three to 17 months later, functional integration of the grafts into the afferent somatosensory pathway was tested using the 2-[14C]deoxyglucose method for estimation of glucose utilization. Grafted rats (n = 8) and control rats (n = 5) with no arterial occlusion were stimulated in the left vibrissal region resulting in an increased glucose utilization in the left trigeminal sensory nucleus and the right ventroposterior nucleus of the thalamus, whereas the same regions in a group (n = 5) of nonstimulated grafted rats were not activated. Glucose uptake in the right somatosensory cortex of control rats was 96 +/- 5 (mean +/- SEM) mumol/100 gm/min. Neocortical grafts consumed less glucose than cortex in control rats but the vibrissae-stimulated group displayed a 110% higher value than the nonstimulated grafted group (32 +/- 5 vs 15 +/- 2, p < 0.05). We conclude that graft glucose metabolism is increased following stimulation of the host somatosensory pathway, which demonstrates that transplanted neurons can be functionally integrated with neural circuitries of the host after an ischemic insult.}},
author = {{Grabowski, Martin and Brundin, Patrik and Johansson, Barbro}},
issn = {{1531-8249}},
language = {{eng}},
number = {{3}},
pages = {{362--368}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Annals of Neurology}},
title = {{Functional integration of cortical grafts placed in brain infarcts of rats}},
url = {{http://dx.doi.org/10.1002/ana.410340310}},
doi = {{10.1002/ana.410340310}},
volume = {{34}},
year = {{1993}},
}