Upregulation of a non-ETA receptor in human arteries in vitro
Adner, Mikael LU ; Erlinge, David LU
; Nilsson, Lars
and Edvinsson, Leif
(1995)
In Journal of Cardiovascular Pharmacology
26(Suppl. 3).
p.314-316
- Abstract
- Receptor turnover may be a crucial part in the physiology of endothelin (ET). Incubation of vessel segments could be a possible method to demonstrate this. The aim was to study contractile responses of human omental arteries to different ET agonists at various periods after incubation at 37 degrees C in 5% CO2 and air. The maximum effect (Emax; percentage of contraction to 60 mM K+ buffer solution) and the potency of ET-1 were unaltered (pD2 = 8.82 +/- 0.06). The selective ETB agonist IRL 1620 demonstrated a negligible Emax in nonincubated segments (2.4 +/- 0.9%). After only 1 day of incubation the Emax was 51 +/- 23%, and it reached 114 +/- 53% after 5 days. The pD2 of IRL 1620 was stable throughout the incubation time (7.23 +/- 0.08).... (More)
- Receptor turnover may be a crucial part in the physiology of endothelin (ET). Incubation of vessel segments could be a possible method to demonstrate this. The aim was to study contractile responses of human omental arteries to different ET agonists at various periods after incubation at 37 degrees C in 5% CO2 and air. The maximum effect (Emax; percentage of contraction to 60 mM K+ buffer solution) and the potency of ET-1 were unaltered (pD2 = 8.82 +/- 0.06). The selective ETB agonist IRL 1620 demonstrated a negligible Emax in nonincubated segments (2.4 +/- 0.9%). After only 1 day of incubation the Emax was 51 +/- 23%, and it reached 114 +/- 53% after 5 days. The pD2 of IRL 1620 was stable throughout the incubation time (7.23 +/- 0.08). ET-3 showed a moderate Emax in nonincubated segments (55 +/- 18%), with a pD2 of 6.68 +/- 0.24. However, subsequent incubation revealed an increase of pD2 to 8.60 +/- 0.20 on the fifth day. The maximum contraction increased to 206 +/- 44%; this is equal to the contraction obtained in paired experiments with ET-1 (215 +/- 18%). These findings indicate modulation of endothelin receptor expression after incubation of vessel segments, and suggest the gradual appearance of a non-ETA receptor. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109146
- author
- Adner, Mikael
LU
; Erlinge, David
LU
; Nilsson, Lars
and Edvinsson, Leif
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Cardiovascular Pharmacology
- volume
- 26
- issue
- Suppl. 3
- pages
- 314 - 316
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:8587399
- ISSN
- 1533-4023
- language
- English
- LU publication?
- yes
- id
- f19f4e8e-760d-4243-84ae-f0a99a85ede7 (old id 1109146)
- date added to LUP
- 2016-04-01 11:33:25
- date last changed
- 2018-11-21 19:57:53
@article{f19f4e8e-760d-4243-84ae-f0a99a85ede7,
abstract = {{Receptor turnover may be a crucial part in the physiology of endothelin (ET). Incubation of vessel segments could be a possible method to demonstrate this. The aim was to study contractile responses of human omental arteries to different ET agonists at various periods after incubation at 37 degrees C in 5% CO2 and air. The maximum effect (Emax; percentage of contraction to 60 mM K+ buffer solution) and the potency of ET-1 were unaltered (pD2 = 8.82 +/- 0.06). The selective ETB agonist IRL 1620 demonstrated a negligible Emax in nonincubated segments (2.4 +/- 0.9%). After only 1 day of incubation the Emax was 51 +/- 23%, and it reached 114 +/- 53% after 5 days. The pD2 of IRL 1620 was stable throughout the incubation time (7.23 +/- 0.08). ET-3 showed a moderate Emax in nonincubated segments (55 +/- 18%), with a pD2 of 6.68 +/- 0.24. However, subsequent incubation revealed an increase of pD2 to 8.60 +/- 0.20 on the fifth day. The maximum contraction increased to 206 +/- 44%; this is equal to the contraction obtained in paired experiments with ET-1 (215 +/- 18%). These findings indicate modulation of endothelin receptor expression after incubation of vessel segments, and suggest the gradual appearance of a non-ETA receptor.}},
author = {{Adner, Mikael and Erlinge, David and Nilsson, Lars and Edvinsson, Leif}},
issn = {{1533-4023}},
language = {{eng}},
number = {{Suppl. 3}},
pages = {{314--316}},
publisher = {{Lippincott Williams & Wilkins}},
series = {{Journal of Cardiovascular Pharmacology}},
title = {{Upregulation of a non-ETA receptor in human arteries in vitro}},
volume = {{26}},
year = {{1995}},
}