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A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus

Olsson, Martin L LU orcid and Chester, Alan LU (1995) In Vox Sanguinis 69(3). p.242-247
Abstract
An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vox Sanguinis
volume
69
issue
3
pages
242 - 247
publisher
Wiley-Blackwell
external identifiers
  • pmid:8578738
  • scopus:0028844274
ISSN
1423-0410
language
English
LU publication?
yes
id
fa562d06-2f99-4f4a-b30e-7b258326c973 (old id 1109592)
date added to LUP
2016-04-01 15:23:35
date last changed
2022-01-25 12:46:42
@article{fa562d06-2f99-4f4a-b30e-7b258326c973,
  abstract     = {{An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single-tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost-efficient and informative ABO genotyping method reported to date.}},
  author       = {{Olsson, Martin L and Chester, Alan}},
  issn         = {{1423-0410}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{242--247}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Vox Sanguinis}},
  title        = {{A rapid and simple ABO genotype screening method using a novel B/O2 versus A/O2 discriminating nucleotide substitution at the ABO locus}},
  volume       = {{69}},
  year         = {{1995}},
}