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Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma

Jönsson, Bo A LU and Åkesson, Bengt LU (1997) In Journal of Chromatography. B 704(1-2). p.151-158
Abstract
A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100... (More)
A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP. (Less)
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type
Contribution to journal
publication status
published
subject
keywords
N-Methylsuccinimide, 2-Hydroxy-N-methylsuccinimide, N-Methyl-2-pyrrolidone
in
Journal of Chromatography. B
volume
704
issue
1-2
pages
151 - 158
publisher
Elsevier
external identifiers
  • pmid:9518144
  • scopus:2542508150
ISSN
1387-2273
DOI
10.1016/S0378-4347(97)00452-0
language
English
LU publication?
yes
id
c5dfd62a-6b6a-4b0c-98e7-4dea203dddd4 (old id 1111493)
date added to LUP
2016-04-01 16:44:31
date last changed
2022-01-28 21:50:25
@article{c5dfd62a-6b6a-4b0c-98e7-4dea203dddd4,
  abstract     = {{A method for determination of N-methylsuccinimide (MSI) and 2-hydroxy-N-methylsuccinimide (2-HMSI) in human urine and of MSI in human plasma was developed. MSI and 2-HMSI are metabolites of the widely used organic solvent N-methyl-2-pyrrolidone (NMP). MSI and 2-HMSI were purified from urine and plasma by C8 solid-phase extraction and analysed by gas chromatography-mass spectrometry in the negative-ion chemical ionisation mode. The intra-day precisions in urine were 2-6% for MSI (50 and 400 ng/ml) and 3-5% for 2-HMSI (1000 and 8000 ng/ml). For MSI in plasma it was 2% (60 and 1200 ng/ml). The between-day precisions in urine were 3-4% for MSI (100 and 1000 ng/ml) and 2-4% for 2-HMSI (10,000 and 18,000 ng/ml) and 3-4% for MSI in plasma (100 and 900 ng/ml). The recoveries from urine were 109-117% for MSI (50 and 400 ng/ml) and 81-89% for 2-HMSI (1000 and 8000 ng/ml). The recovery of MSI from plasma was 91-101% (50 and 500 ng/ml). The detection limits for MSI were 3 ng/ml in urine and 1 ng/ml in plasma and that of 2-HMSI in urine was 200 ng/ml. The method is applicable for analysis of urine and plasma samples from workers exposed to NMP.}},
  author       = {{Jönsson, Bo A and Åkesson, Bengt}},
  issn         = {{1387-2273}},
  keywords     = {{N-Methylsuccinimide; 2-Hydroxy-N-methylsuccinimide; N-Methyl-2-pyrrolidone}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{151--158}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Determination of N-methylsuccinimide and 2-hydroxy-N-methylsuccinimide in human urine and plasma}},
  url          = {{http://dx.doi.org/10.1016/S0378-4347(97)00452-0}},
  doi          = {{10.1016/S0378-4347(97)00452-0}},
  volume       = {{704}},
  year         = {{1997}},
}