Inhibition of calcium entry preserves contractility of arterial smooth muscle in culture
Lindqvist, Anders LU ; Nilsson, Bengt-Olof LU
and Hellstrand, Per
LU
(1997)
In Journal of Vascular Research
34(2).
p.103-108
- Abstract
- The addition of the growth stimulator fetal calf serum (FCS, 10%) to rings of rat tail artery causes an increase in [Ca2+]i, accompanied by contraction. This response was inhibited by the calcium entry blocker verapamil (1 microM). To investigate the effect of Ca2+ entry blockade on growth and contractility, rings of rat tail artery were cultured for 4 days in medium with or without FCS and then mounted for tension registration and stimulated with noradrenaline (NA) or high-K+ solution. In cultured rings growth was quantitated by [3H]-thymidine incorporation and increase in protein contents. FCS in the medium stimulated DNA synthesis by about 2-fold and increased protein contents by about 70%. The growth-stimulated cultured rings developed... (More)
- The addition of the growth stimulator fetal calf serum (FCS, 10%) to rings of rat tail artery causes an increase in [Ca2+]i, accompanied by contraction. This response was inhibited by the calcium entry blocker verapamil (1 microM). To investigate the effect of Ca2+ entry blockade on growth and contractility, rings of rat tail artery were cultured for 4 days in medium with or without FCS and then mounted for tension registration and stimulated with noradrenaline (NA) or high-K+ solution. In cultured rings growth was quantitated by [3H]-thymidine incorporation and increase in protein contents. FCS in the medium stimulated DNA synthesis by about 2-fold and increased protein contents by about 70%. The growth-stimulated cultured rings developed less force than freshly prepared rings (2.2 +/- 0.3 vs. 8.3 +/- 1.0 mN/mm). The addition of 1 microM verapamil to the medium during culture increased maximal NA-evoked force to 5.0 +/- 0.4 mN/mm but had no effect on the increases in DNA synthesis and protein contents. Force developed by growth-arrested rings, cultured in the absence of FCS, was not different from that of freshly prepared rings (7.2 +/- 0.6 mM/mm). Verapamil did not affect maximal force in these rings. Similar responses were seen when contraction was elicited by high-K+ solution. We conclude that verapamil, present during culture, preserves contractility of arterial smooth muscle, and that this effect is not parallel to inhibition of growth. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111985
- author
- Lindqvist, Anders
LU
; Nilsson, Bengt-Olof
LU
and Hellstrand, Per
LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Vascular Research
- volume
- 34
- issue
- 2
- pages
- 103 - 108
- publisher
- Karger
- external identifiers
-
- pmid:9167642
- scopus:0030987013
- ISSN
- 1423-0135
- language
- English
- LU publication?
- yes
- id
- fd716da4-ccc4-44a1-9266-bc9570e83a34 (old id 1111985)
- date added to LUP
- 2016-04-01 15:54:08
- date last changed
- 2022-01-28 07:55:02
@article{fd716da4-ccc4-44a1-9266-bc9570e83a34,
abstract = {{The addition of the growth stimulator fetal calf serum (FCS, 10%) to rings of rat tail artery causes an increase in [Ca2+]i, accompanied by contraction. This response was inhibited by the calcium entry blocker verapamil (1 microM). To investigate the effect of Ca2+ entry blockade on growth and contractility, rings of rat tail artery were cultured for 4 days in medium with or without FCS and then mounted for tension registration and stimulated with noradrenaline (NA) or high-K+ solution. In cultured rings growth was quantitated by [3H]-thymidine incorporation and increase in protein contents. FCS in the medium stimulated DNA synthesis by about 2-fold and increased protein contents by about 70%. The growth-stimulated cultured rings developed less force than freshly prepared rings (2.2 +/- 0.3 vs. 8.3 +/- 1.0 mN/mm). The addition of 1 microM verapamil to the medium during culture increased maximal NA-evoked force to 5.0 +/- 0.4 mN/mm but had no effect on the increases in DNA synthesis and protein contents. Force developed by growth-arrested rings, cultured in the absence of FCS, was not different from that of freshly prepared rings (7.2 +/- 0.6 mM/mm). Verapamil did not affect maximal force in these rings. Similar responses were seen when contraction was elicited by high-K+ solution. We conclude that verapamil, present during culture, preserves contractility of arterial smooth muscle, and that this effect is not parallel to inhibition of growth.}},
author = {{Lindqvist, Anders and Nilsson, Bengt-Olof and Hellstrand, Per}},
issn = {{1423-0135}},
language = {{eng}},
number = {{2}},
pages = {{103--108}},
publisher = {{Karger}},
series = {{Journal of Vascular Research}},
title = {{Inhibition of calcium entry preserves contractility of arterial smooth muscle in culture}},
volume = {{34}},
year = {{1997}},
}