Association between inflammation and epithelial damage-restitution processes in allergic airways in vivo
(1997) In Clinical and Experimental Allergy 27(11). p.1344-1355- Abstract
- BACKGROUND: Associations between allergen challenge-induced sites of epithelial damage and the distribution of leucocytes and extravasated plasma remain unexplored. OBJECTIVE: To study neutrophils, eosinophils, and fibrinogen at allergen challenge-induced patchy epithelial damage-restitution sites in guinea-pig trachea. METHODS: After local challenge tracheal tissue (cryo sections and whole-mounts) and lumen (selective tracheal lavage) were examined at 1, 5, and 24 h. Eosinophils, neutrophils and fibrinogen were identified by histochemistry. RESULTS: Neutrophils increased markedly in tracheal lavage fluids and in tissue and were strongly associated with the challenge-induced epithelial craters of damage-restitution. At 1 and 24 h... (More)
- BACKGROUND: Associations between allergen challenge-induced sites of epithelial damage and the distribution of leucocytes and extravasated plasma remain unexplored. OBJECTIVE: To study neutrophils, eosinophils, and fibrinogen at allergen challenge-induced patchy epithelial damage-restitution sites in guinea-pig trachea. METHODS: After local challenge tracheal tissue (cryo sections and whole-mounts) and lumen (selective tracheal lavage) were examined at 1, 5, and 24 h. Eosinophils, neutrophils and fibrinogen were identified by histochemistry. RESULTS: Neutrophils increased markedly in tracheal lavage fluids and in tissue and were strongly associated with the challenge-induced epithelial craters of damage-restitution. At 1 and 24 h eosinophils were increased in the tracheal lumen whereas the surrounding tissue displayed a reversed pattern. Gels rich in fibrinogen, neutrophils, and eosinophils were present in epithelial crater areas, protruding into the lumen. Clusters of free eosinophil granules, Cfegs, released through lysis of eosinophils, and neutrophils with long cytoplasmatic protrusions abounded in these crater areas. CONCLUSION: The present findings provide important new insights into allergic airways where sites of epithelial damage-restitution processes emerge as the major loci for eosinophil, neutrophil, and plasma protein activities, the latter likely causing leukocyte adhesion and activation in vivo. The distribution of eosinophils in this study suggests roles of these cells both in airway mucosa and in regional lymph nodes. Based on the present study we also propose that lysis of eosinophils and Cfegs generation are a major paradigm for activation of these cells in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112500
- author
- Erjefält, Jonas LU ; Korsgren, Magnus LU ; Nilsson, M C ; Sundler, Frank LU and Persson, Carl LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- shedding, neutrophils, eosinophils, allergen, airways
- in
- Clinical and Experimental Allergy
- volume
- 27
- issue
- 11
- pages
- 1344 - 1355
- publisher
- Wiley
- external identifiers
-
- pmid:9420140
- scopus:0030718616
- ISSN
- 1365-2222
- DOI
- 10.1111/j.1365-2222.1997.tb01181.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Neuroendocrine Cell Biology (013212008), Airway Inflammation and Immunology (013212038)
- id
- 4b9a5d58-cf65-48de-acb9-081ddc005328 (old id 1112500)
- date added to LUP
- 2016-04-01 11:52:42
- date last changed
- 2022-01-26 19:37:06
@article{4b9a5d58-cf65-48de-acb9-081ddc005328,
abstract = {{BACKGROUND: Associations between allergen challenge-induced sites of epithelial damage and the distribution of leucocytes and extravasated plasma remain unexplored. OBJECTIVE: To study neutrophils, eosinophils, and fibrinogen at allergen challenge-induced patchy epithelial damage-restitution sites in guinea-pig trachea. METHODS: After local challenge tracheal tissue (cryo sections and whole-mounts) and lumen (selective tracheal lavage) were examined at 1, 5, and 24 h. Eosinophils, neutrophils and fibrinogen were identified by histochemistry. RESULTS: Neutrophils increased markedly in tracheal lavage fluids and in tissue and were strongly associated with the challenge-induced epithelial craters of damage-restitution. At 1 and 24 h eosinophils were increased in the tracheal lumen whereas the surrounding tissue displayed a reversed pattern. Gels rich in fibrinogen, neutrophils, and eosinophils were present in epithelial crater areas, protruding into the lumen. Clusters of free eosinophil granules, Cfegs, released through lysis of eosinophils, and neutrophils with long cytoplasmatic protrusions abounded in these crater areas. CONCLUSION: The present findings provide important new insights into allergic airways where sites of epithelial damage-restitution processes emerge as the major loci for eosinophil, neutrophil, and plasma protein activities, the latter likely causing leukocyte adhesion and activation in vivo. The distribution of eosinophils in this study suggests roles of these cells both in airway mucosa and in regional lymph nodes. Based on the present study we also propose that lysis of eosinophils and Cfegs generation are a major paradigm for activation of these cells in vivo.}},
author = {{Erjefält, Jonas and Korsgren, Magnus and Nilsson, M C and Sundler, Frank and Persson, Carl}},
issn = {{1365-2222}},
keywords = {{shedding; neutrophils; eosinophils; allergen; airways}},
language = {{eng}},
number = {{11}},
pages = {{1344--1355}},
publisher = {{Wiley}},
series = {{Clinical and Experimental Allergy}},
title = {{Association between inflammation and epithelial damage-restitution processes in allergic airways in vivo}},
url = {{http://dx.doi.org/10.1111/j.1365-2222.1997.tb01181.x}},
doi = {{10.1111/j.1365-2222.1997.tb01181.x}},
volume = {{27}},
year = {{1997}},
}