Effects of topical platelet activating factor on the guinea-pig tracheobronchial mucosa in vivo
(1997) In Acta Physiologica Scandinavica 160(4). p.387-391- Abstract
- Platelet activating factor (PAF) has been reported to produce a variety of airway effects including epithelial damage and increased airway-lung absorption of hydrophilic tracers. The present study examines effects of PAF on the guinea-pig tracheobronchial mucosa in vivo. Vehicle with and without PAF (4.0 and 8.0 nmol) was superfused onto the tracheobronchial mucosa. The levels of 125I-albumin, previously given intravenously, were determined in tracheobronchial lavage fluids as an index of mucosal exudation of plasma. The mucosa was also examined by scanning electron microscopy. In separate animals, 99mTc-DTPA (a low molecular weight, 492 Da, hydrophilic tracer) was superfused onto the mucosal surface through an oro-tracheal catheter,... (More)
- Platelet activating factor (PAF) has been reported to produce a variety of airway effects including epithelial damage and increased airway-lung absorption of hydrophilic tracers. The present study examines effects of PAF on the guinea-pig tracheobronchial mucosa in vivo. Vehicle with and without PAF (4.0 and 8.0 nmol) was superfused onto the tracheobronchial mucosa. The levels of 125I-albumin, previously given intravenously, were determined in tracheobronchial lavage fluids as an index of mucosal exudation of plasma. The mucosa was also examined by scanning electron microscopy. In separate animals, 99mTc-DTPA (a low molecular weight, 492 Da, hydrophilic tracer) was superfused onto the mucosal surface through an oro-tracheal catheter, together with vehicle or PAF (8.0 nmol). A gamma camera determined the disappearance rate of 99mTc-DTPA from the airways as an index of mucosal absorption. PAF produced dose-dependent mucosal exudation of plasma up to 20-fold greater than control (P < 0.001). However, PAF did not damage the epithelium and the absorption ability of the airway mucosa was unaffected. The results, in contrast to previous reports, suggest that PAF may not readily damage the airway mucosa even at large exudative doses of the agent. The present finding support the view that the plasticity of the epithelial junctions allows the creation of valve-like paracellular pathways for unidirectional clearance of extravasated plasma into the airway lumen. We suggest that endogenous PAF may participate in first line respiratory defence reactions by causing lumenal entry of bulk plasma without harming the epithelium. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112505
- author
- Greiff, Lennart LU ; Erjefalt, I ; Erjefält, Jonas LU ; Wollmer, Per LU ; Sundler, Frank LU and Persson, Carl LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ultrastructure, plasma exudation, inflammation, absorption, airway
- in
- Acta Physiologica Scandinavica
- volume
- 160
- issue
- 4
- pages
- 387 - 391
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:9338520
- scopus:0030790148
- ISSN
- 0001-6772
- DOI
- 10.1046/j.1365-201X.1997.00183.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Otorhinolaryngology (Lund) (013044000), Clinical Physiology and Nuclear Medicine Unit (013242320), Neuroendocrine Cell Biology (013212008), Airway Inflammation and Immunology (013212038)
- id
- ec2ba398-980d-4046-baed-14f1ad4280fd (old id 1112505)
- date added to LUP
- 2016-04-01 16:44:38
- date last changed
- 2023-09-05 00:27:36
@article{ec2ba398-980d-4046-baed-14f1ad4280fd,
abstract = {{Platelet activating factor (PAF) has been reported to produce a variety of airway effects including epithelial damage and increased airway-lung absorption of hydrophilic tracers. The present study examines effects of PAF on the guinea-pig tracheobronchial mucosa in vivo. Vehicle with and without PAF (4.0 and 8.0 nmol) was superfused onto the tracheobronchial mucosa. The levels of 125I-albumin, previously given intravenously, were determined in tracheobronchial lavage fluids as an index of mucosal exudation of plasma. The mucosa was also examined by scanning electron microscopy. In separate animals, 99mTc-DTPA (a low molecular weight, 492 Da, hydrophilic tracer) was superfused onto the mucosal surface through an oro-tracheal catheter, together with vehicle or PAF (8.0 nmol). A gamma camera determined the disappearance rate of 99mTc-DTPA from the airways as an index of mucosal absorption. PAF produced dose-dependent mucosal exudation of plasma up to 20-fold greater than control (P < 0.001). However, PAF did not damage the epithelium and the absorption ability of the airway mucosa was unaffected. The results, in contrast to previous reports, suggest that PAF may not readily damage the airway mucosa even at large exudative doses of the agent. The present finding support the view that the plasticity of the epithelial junctions allows the creation of valve-like paracellular pathways for unidirectional clearance of extravasated plasma into the airway lumen. We suggest that endogenous PAF may participate in first line respiratory defence reactions by causing lumenal entry of bulk plasma without harming the epithelium.}},
author = {{Greiff, Lennart and Erjefalt, I and Erjefält, Jonas and Wollmer, Per and Sundler, Frank and Persson, Carl}},
issn = {{0001-6772}},
keywords = {{ultrastructure; plasma exudation; inflammation; absorption; airway}},
language = {{eng}},
number = {{4}},
pages = {{387--391}},
publisher = {{Wiley-Blackwell}},
series = {{Acta Physiologica Scandinavica}},
title = {{Effects of topical platelet activating factor on the guinea-pig tracheobronchial mucosa in vivo}},
url = {{http://dx.doi.org/10.1046/j.1365-201X.1997.00183.x}},
doi = {{10.1046/j.1365-201X.1997.00183.x}},
volume = {{160}},
year = {{1997}},
}