Various phosphodiesterase subtypes mediate the in vivo antilipolytic effect of insulin on adipose tissue and skeletal muscle in man
Enoksson, S ; Degerman, Eva LU
; Hagstrom-Toft, E
; Large, V
and Arner, P
(1998)
In Diabetologia
41(5).
p.560-568
- Abstract
- The antilipolytic effect of insulin on human abdominal subcutaneous adipose tissue and skeletal muscle during local inhibition of cAMP-phosphodiesterases (PDEs) was investigated in vivo, by combining microdialysis with a euglycaemic, hyperinsulinaemic clamp. During hyperinsulinaemia, the glycerol concentration decreased by 40% in fat and by 33% in muscle. Addition of the selective PDE3-inhibitor amrinone abolished the insulin-induced decrease in adipose glycerol concentration, but did not influence the glycerol concentration in skeletal muscle. Nor did the PDE4-selective inhibitor rolipram or the PDE5-selective inhibitor dipyridamole influence the insulin-induced decrease in muscle tissue glycerol. However, the non-selective PDE-inhibitor... (More)
- The antilipolytic effect of insulin on human abdominal subcutaneous adipose tissue and skeletal muscle during local inhibition of cAMP-phosphodiesterases (PDEs) was investigated in vivo, by combining microdialysis with a euglycaemic, hyperinsulinaemic clamp. During hyperinsulinaemia, the glycerol concentration decreased by 40% in fat and by 33% in muscle. Addition of the selective PDE3-inhibitor amrinone abolished the insulin-induced decrease in adipose glycerol concentration, but did not influence the glycerol concentration in skeletal muscle. Nor did the PDE4-selective inhibitor rolipram or the PDE5-selective inhibitor dipyridamole influence the insulin-induced decrease in muscle tissue glycerol. However, the non-selective PDE-inhibitor theophylline counteracted the antilipolytic action of insulin at both sites. The specific activity of PDEs was also determined in both tissues. PDE3-activity was 36.8+/-6.4 pmol x min(-1) x mg(-1) in adipose tissue and 3.9+/-0.5 pmol x min(-1) x mg(-1) in muscle. PDE4-activity in skeletal muscle was high, i.e., 60.7+/-10.2 pmol x min(-1) x mg(-1) but 8.5 pmol x min(-1) x mg(-1) or less in adipose tissue. In conclusion, insulin inhibits lipolysis in adipose tissue and skeletal muscle by activation of different PDEs, suggesting a unique metabolic role of muscle lipolysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1113559
- author
- Enoksson, S
; Degerman, Eva
LU
; Hagstrom-Toft, E
; Large, V
and Arner, P
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Microdialysis, glycerol, interstitial flow, phosphodiesterase inhibitors
- in
- Diabetologia
- volume
- 41
- issue
- 5
- pages
- 560 - 568
- publisher
- Springer
- external identifiers
-
- pmid:9628274
- scopus:0031968221
- ISSN
- 1432-0428
- DOI
- 10.1007/s001250050947
- language
- English
- LU publication?
- yes
- id
- 877c87ba-990d-4d23-aaaf-30f37c47b19c (old id 1113559)
- date added to LUP
- 2016-04-01 12:31:26
- date last changed
- 2022-04-21 08:38:12
@article{877c87ba-990d-4d23-aaaf-30f37c47b19c,
abstract = {{The antilipolytic effect of insulin on human abdominal subcutaneous adipose tissue and skeletal muscle during local inhibition of cAMP-phosphodiesterases (PDEs) was investigated in vivo, by combining microdialysis with a euglycaemic, hyperinsulinaemic clamp. During hyperinsulinaemia, the glycerol concentration decreased by 40% in fat and by 33% in muscle. Addition of the selective PDE3-inhibitor amrinone abolished the insulin-induced decrease in adipose glycerol concentration, but did not influence the glycerol concentration in skeletal muscle. Nor did the PDE4-selective inhibitor rolipram or the PDE5-selective inhibitor dipyridamole influence the insulin-induced decrease in muscle tissue glycerol. However, the non-selective PDE-inhibitor theophylline counteracted the antilipolytic action of insulin at both sites. The specific activity of PDEs was also determined in both tissues. PDE3-activity was 36.8+/-6.4 pmol x min(-1) x mg(-1) in adipose tissue and 3.9+/-0.5 pmol x min(-1) x mg(-1) in muscle. PDE4-activity in skeletal muscle was high, i.e., 60.7+/-10.2 pmol x min(-1) x mg(-1) but 8.5 pmol x min(-1) x mg(-1) or less in adipose tissue. In conclusion, insulin inhibits lipolysis in adipose tissue and skeletal muscle by activation of different PDEs, suggesting a unique metabolic role of muscle lipolysis.}},
author = {{Enoksson, S and Degerman, Eva and Hagstrom-Toft, E and Large, V and Arner, P}},
issn = {{1432-0428}},
keywords = {{Microdialysis; glycerol; interstitial flow; phosphodiesterase inhibitors}},
language = {{eng}},
number = {{5}},
pages = {{560--568}},
publisher = {{Springer}},
series = {{Diabetologia}},
title = {{Various phosphodiesterase subtypes mediate the in vivo antilipolytic effect of insulin on adipose tissue and skeletal muscle in man}},
url = {{http://dx.doi.org/10.1007/s001250050947}},
doi = {{10.1007/s001250050947}},
volume = {{41}},
year = {{1998}},
}