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Mucosal T lymphocyte numbers are selectively reduced in integrin alpha E (CD103)-deficient mice

Schon, M P ; Arya, A ; Murphy, E A ; Adams, C M ; Strauch, U G ; Agace, William LU ; Marsal, J ; Donohue, J P ; Her, H and Beier, D R , et al. (1999) In Journal of Immunology 162(11). p.6641-6649
Abstract
The mucosal lymphocyte integrin alpha E(CD103)beta 7 is thought to be important for intraepithelial lymphocyte (IEL) localization or function. We cloned the murine integrin gene encoding alpha E, localized it to chromosome 11, and generated integrin alpha E-deficient mice. In alpha E-/- mice, intestinal and vaginal IEL numbers were reduced, consistent with the known binding of alpha E beta 7 to E-cadherin expressed on epithelial cells. However, it was surprising that lamina propria T lymphocyte numbers were diminished, as E-cadherin is not expressed in the lamina propria. In contrast, peribronchial, intrapulmonary, Peyer's patch, and splenic T lymphocyte numbers were not reduced in alpha E-deficient mice. Thus, alpha E beta 7 was important... (More)
The mucosal lymphocyte integrin alpha E(CD103)beta 7 is thought to be important for intraepithelial lymphocyte (IEL) localization or function. We cloned the murine integrin gene encoding alpha E, localized it to chromosome 11, and generated integrin alpha E-deficient mice. In alpha E-/- mice, intestinal and vaginal IEL numbers were reduced, consistent with the known binding of alpha E beta 7 to E-cadherin expressed on epithelial cells. However, it was surprising that lamina propria T lymphocyte numbers were diminished, as E-cadherin is not expressed in the lamina propria. In contrast, peribronchial, intrapulmonary, Peyer's patch, and splenic T lymphocyte numbers were not reduced in alpha E-deficient mice. Thus, alpha E beta 7 was important for generating or maintaining the gut and vaginal T lymphocytes located diffusely within the epithelium or lamina propria but not for generating the gut-associated organized lymphoid tissues. Finally, the impact of alpha E deficiency upon intestinal IEL numbers was greater at 3-4 wk of life than in younger animals, and affected the TCR alpha beta+ CD8+ T cells more than the gamma delta T cells or the TCR alpha beta+ CD4+CD8- population. These findings suggest that alpha E beta 7 is involved in the expansion/recruitment of TCR alpha beta+ CD8+ IEL following microbial colonization. Integrin alpha E-deficient mice will provide an important tool for studying the role of alpha E beta 7 and of alpha E beta 7-expressing mucosal T lymphocytes in vivo. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Immunology
volume
162
issue
11
pages
6641 - 6649
publisher
American Association of Immunologists
external identifiers
  • pmid:10352281
ISSN
1550-6606
language
English
LU publication?
yes
id
c33adcca-97f4-4a08-bdba-9710976954e4 (old id 1114203)
alternative location
http://www.jimmunol.org/cgi/content/full/162/11/6641
date added to LUP
2016-04-01 16:24:49
date last changed
2018-11-21 20:41:13
@article{c33adcca-97f4-4a08-bdba-9710976954e4,
  abstract     = {{The mucosal lymphocyte integrin alpha E(CD103)beta 7 is thought to be important for intraepithelial lymphocyte (IEL) localization or function. We cloned the murine integrin gene encoding alpha E, localized it to chromosome 11, and generated integrin alpha E-deficient mice. In alpha E-/- mice, intestinal and vaginal IEL numbers were reduced, consistent with the known binding of alpha E beta 7 to E-cadherin expressed on epithelial cells. However, it was surprising that lamina propria T lymphocyte numbers were diminished, as E-cadherin is not expressed in the lamina propria. In contrast, peribronchial, intrapulmonary, Peyer's patch, and splenic T lymphocyte numbers were not reduced in alpha E-deficient mice. Thus, alpha E beta 7 was important for generating or maintaining the gut and vaginal T lymphocytes located diffusely within the epithelium or lamina propria but not for generating the gut-associated organized lymphoid tissues. Finally, the impact of alpha E deficiency upon intestinal IEL numbers was greater at 3-4 wk of life than in younger animals, and affected the TCR alpha beta+ CD8+ T cells more than the gamma delta T cells or the TCR alpha beta+ CD4+CD8- population. These findings suggest that alpha E beta 7 is involved in the expansion/recruitment of TCR alpha beta+ CD8+ IEL following microbial colonization. Integrin alpha E-deficient mice will provide an important tool for studying the role of alpha E beta 7 and of alpha E beta 7-expressing mucosal T lymphocytes in vivo.}},
  author       = {{Schon, M P and Arya, A and Murphy, E A and Adams, C M and Strauch, U G and Agace, William and Marsal, J and Donohue, J P and Her, H and Beier, D R and Olson, S and Lefrancois, L and Brenner, M B and Grusby, M J and Parker, C M}},
  issn         = {{1550-6606}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{6641--6649}},
  publisher    = {{American Association of Immunologists}},
  series       = {{Journal of Immunology}},
  title        = {{Mucosal T lymphocyte numbers are selectively reduced in integrin alpha E (CD103)-deficient mice}},
  url          = {{http://www.jimmunol.org/cgi/content/full/162/11/6641}},
  volume       = {{162}},
  year         = {{1999}},
}