Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure
Hou, Mingyan ; Malmsjö, Malin LU ; Moller, Sebastian ; Pantev, Emil LU ; Bergdahl, Anders ; Zhao, X H ; Sun, X Y ; Hedner, T ; Edvinsson, Lars LU and Erlinge, David LU
(1999)
In Life Sciences
65(11).
p.1195-1206
- Abstract
- We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A... (More)
- We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A prominent change was seen for the P2X1- and P2Y2-receptor mRNA levels which were increased 2.7-fold and 4.7-fold respectively in the myocardium from the left ventricle of CHF-rats. In contrast, the P2Y1-, P2Y4- and P2Y6-receptor mRNA levels were not significantly altered in CHF rats. In human myocard the P2X1-, P2Y1-, P2Y2-, P2Y6- and P2Y11-receptors were detected by RT-PCR in both right and left atria and ventricles, while the P2Y4-receptor band was weak or absent. In conclusion, most of the studied P2-receptors were expressed in both rat and human hearts. Furthermore, the P2X1- and P2Y2-receptor mRNA were upregulated in CHF, suggesting a pathophysiological role for these receptors in the development of heart failure. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1114807
- author
- Hou, Mingyan
; Malmsjö, Malin
LU
; Moller, Sebastian
; Pantev, Emil
LU
; Bergdahl, Anders
; Zhao, X H
; Sun, X Y
; Hedner, T
; Edvinsson, Lars
LU
and Erlinge, David
LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- P2Y, P2X, receptor, quantitative RT-PCR, congestive heart failure
- in
- Life Sciences
- volume
- 65
- issue
- 11
- pages
- 1195 - 1206
- publisher
- Elsevier
- external identifiers
-
- pmid:10503935
- scopus:0033529419
- ISSN
- 1879-0631
- DOI
- 10.1016/S0024-3205(99)00353-7
- language
- English
- LU publication?
- yes
- id
- 6912f71d-035f-40e5-a295-a74d687ae913 (old id 1114807)
- date added to LUP
- 2016-04-01 12:18:46
- date last changed
- 2024-01-08 16:02:57
@article{6912f71d-035f-40e5-a295-a74d687ae913,
abstract = {{We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A prominent change was seen for the P2X1- and P2Y2-receptor mRNA levels which were increased 2.7-fold and 4.7-fold respectively in the myocardium from the left ventricle of CHF-rats. In contrast, the P2Y1-, P2Y4- and P2Y6-receptor mRNA levels were not significantly altered in CHF rats. In human myocard the P2X1-, P2Y1-, P2Y2-, P2Y6- and P2Y11-receptors were detected by RT-PCR in both right and left atria and ventricles, while the P2Y4-receptor band was weak or absent. In conclusion, most of the studied P2-receptors were expressed in both rat and human hearts. Furthermore, the P2X1- and P2Y2-receptor mRNA were upregulated in CHF, suggesting a pathophysiological role for these receptors in the development of heart failure.}},
author = {{Hou, Mingyan and Malmsjö, Malin and Moller, Sebastian and Pantev, Emil and Bergdahl, Anders and Zhao, X H and Sun, X Y and Hedner, T and Edvinsson, Lars and Erlinge, David}},
issn = {{1879-0631}},
keywords = {{P2Y; P2X; receptor; quantitative RT-PCR; congestive heart failure}},
language = {{eng}},
number = {{11}},
pages = {{1195--1206}},
publisher = {{Elsevier}},
series = {{Life Sciences}},
title = {{Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure}},
url = {{http://dx.doi.org/10.1016/S0024-3205(99)00353-7}},
doi = {{10.1016/S0024-3205(99)00353-7}},
volume = {{65}},
year = {{1999}},
}