Tissue distribution of the lipocalin alpha-1 microglobulin in the developing human fetus
(2000) In Journal of Histochemistry and Cytochemistry 48(11). p.1545-1552- Abstract
- Alpha-1 microglobulin (alpha(1)m), a lipocalin, is an evolutionarily conserved immunomodulatory plasma protein. In all species studied, alpha(1)m is synthesized by hepatocytes and catabolized in the renal proximal tubular cells. alpha(1)m deficiency has not been reported in any species, suggesting that its absence is lethal and indicating an important physiological role for this protein To clarify its functional role, tissue distribution studies are crucial. Such studies in humans have been restricted largely to adult fresh/frozen tissue. Formalin-fixed, paraffin-embedded multi-organ block tissue from aborted fetuses (gestational age range 7-22 weeks) was immunohistochemically examined for alpha(1)m reactivity. Moderate to strong... (More)
- Alpha-1 microglobulin (alpha(1)m), a lipocalin, is an evolutionarily conserved immunomodulatory plasma protein. In all species studied, alpha(1)m is synthesized by hepatocytes and catabolized in the renal proximal tubular cells. alpha(1)m deficiency has not been reported in any species, suggesting that its absence is lethal and indicating an important physiological role for this protein To clarify its functional role, tissue distribution studies are crucial. Such studies in humans have been restricted largely to adult fresh/frozen tissue. Formalin-fixed, paraffin-embedded multi-organ block tissue from aborted fetuses (gestational age range 7-22 weeks) was immunohistochemically examined for alpha(1)m reactivity. Moderate to strong reactivity was seen at all ages in hepatocytes, renal proximal tubule cells, and a subset of pancreatic islet cells. Muscle (cardiac, skeletal, or smooth), adrenal cortex, a scattered subset of intestinal mucosal cells, tips of small intestinal villi, and Leydig cells showed weaker and/or variable levels of reactivity. Connective tissue stained with variable location and intensity. The following cells/sites were consistently negative: thymus, spleen, hematopoietic cells, lung parenchyma, glomeruli, exocrine pancreas, epidermis, cartilage/bone, ovary, seminiferous tubules, epididymis, thyroid, and parathyroid. The results underscore the dominant role of liver and kidney in fetal alpha(1)m metabolism and provide a framework for understanding the functional role of this immunoregulatory protein. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117732
- author
- Logdberg, L E ; Åkerström, Bo LU and Badve, S
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Histochemistry and Cytochemistry
- volume
- 48
- issue
- 11
- pages
- 1545 - 1552
- publisher
- Histochemical Society
- external identifiers
-
- pmid:11036097
- scopus:0033766519
- ISSN
- 0022-1554
- language
- English
- LU publication?
- yes
- id
- e61f241e-1f9b-4a6c-a580-f9429c395ec8 (old id 1117732)
- alternative location
- http://www.jhc.org/cgi/content/abstract/48/11/1545
- date added to LUP
- 2016-04-01 16:44:06
- date last changed
- 2022-01-28 21:44:58
@article{e61f241e-1f9b-4a6c-a580-f9429c395ec8,
abstract = {{Alpha-1 microglobulin (alpha(1)m), a lipocalin, is an evolutionarily conserved immunomodulatory plasma protein. In all species studied, alpha(1)m is synthesized by hepatocytes and catabolized in the renal proximal tubular cells. alpha(1)m deficiency has not been reported in any species, suggesting that its absence is lethal and indicating an important physiological role for this protein To clarify its functional role, tissue distribution studies are crucial. Such studies in humans have been restricted largely to adult fresh/frozen tissue. Formalin-fixed, paraffin-embedded multi-organ block tissue from aborted fetuses (gestational age range 7-22 weeks) was immunohistochemically examined for alpha(1)m reactivity. Moderate to strong reactivity was seen at all ages in hepatocytes, renal proximal tubule cells, and a subset of pancreatic islet cells. Muscle (cardiac, skeletal, or smooth), adrenal cortex, a scattered subset of intestinal mucosal cells, tips of small intestinal villi, and Leydig cells showed weaker and/or variable levels of reactivity. Connective tissue stained with variable location and intensity. The following cells/sites were consistently negative: thymus, spleen, hematopoietic cells, lung parenchyma, glomeruli, exocrine pancreas, epidermis, cartilage/bone, ovary, seminiferous tubules, epididymis, thyroid, and parathyroid. The results underscore the dominant role of liver and kidney in fetal alpha(1)m metabolism and provide a framework for understanding the functional role of this immunoregulatory protein.}},
author = {{Logdberg, L E and Åkerström, Bo and Badve, S}},
issn = {{0022-1554}},
language = {{eng}},
number = {{11}},
pages = {{1545--1552}},
publisher = {{Histochemical Society}},
series = {{Journal of Histochemistry and Cytochemistry}},
title = {{Tissue distribution of the lipocalin alpha-1 microglobulin in the developing human fetus}},
url = {{http://www.jhc.org/cgi/content/abstract/48/11/1545}},
volume = {{48}},
year = {{2000}},
}