Neural regeneration along longitudinal polyglactin sutures across short and extended defects in the rat sciatic nerve
Scherman, Peter LU ; Lundborg, Göran LU ; Kanje, Martin LU and Dahlin, Lars LU
(2001)
In Journal of Neurosurgery
95(2).
p.316-323
- Abstract
- Object. The authors have previously shown that longitudinal sutures without artificial tube support regeneration across a 7-mm gap in the rat sciatic nerve. In the present study, the authors, compared this new approach with the use of autologous nerve grafts across short defects and examined whether the approach could be used to support regeneration across extended gaps and whether the interposition of a short nerve segment (the stepping-stone procedure) was applicable in this model. Methods. Longitudinal sutures were used to bridge 7- and 15-mm gaps in the rat sciatic nerve. Contralateral comparisons were made to nerve autografts in the 7-mm group and to sutures plus a short interposed nerve segment in the 15-mm group. Regeneration was... (More)
- Object. The authors have previously shown that longitudinal sutures without artificial tube support regeneration across a 7-mm gap in the rat sciatic nerve. In the present study, the authors, compared this new approach with the use of autologous nerve grafts across short defects and examined whether the approach could be used to support regeneration across extended gaps and whether the interposition of a short nerve segment (the stepping-stone procedure) was applicable in this model. Methods. Longitudinal sutures were used to bridge 7- and 15-mm gaps in the rat sciatic nerve. Contralateral comparisons were made to nerve autografts in the 7-mm group and to sutures plus a short interposed nerve segment in the 15-mm group. Regeneration was evaluated at 2, 4, and 12 weeks by using immunocytochemical analysis for Schwann cells, neurofilament protein, and macrophages and at 12 weeks also by using histological examination, including morphometry in the distal tibial trunk and tetanic force measurements in the gastrocnemius muscle. Conclusions. The authors found that the results of regeneration after repair with longitudinal polyglactin sutures across short defects were not significantly different from those produced by the use of autologous nerve grafts. Regeneration, although poor, occurred along sutures across extended gaps and was significantly enhanced by an interposed nerve segment acting as a Schwann cell resource in this model. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1119019
- author
- Scherman, Peter
LU
; Lundborg, Göran
LU
; Kanje, Martin
LU
and Dahlin, Lars
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- nerve regeneration, absorbable suture, nerve graft, interposed nerve segment, Schwann cell, muscle force, nerve fiber
- in
- Journal of Neurosurgery
- volume
- 95
- issue
- 2
- pages
- 316 - 323
- publisher
- American Association of Neurosurgeons
- external identifiers
-
- wos:000170254500019
- scopus:0034860060
- ISSN
- 0022-3085
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Functional Zoology (432112239), Reconstructive Surgery (013240300), Hand Surgery Research Group (013241910)
- id
- cfc6b324-f982-4f64-8348-813b86ae27f2 (old id 1119019)
- date added to LUP
- 2016-04-01 15:54:09
- date last changed
- 2022-01-28 07:55:03
@article{cfc6b324-f982-4f64-8348-813b86ae27f2,
abstract = {{Object. The authors have previously shown that longitudinal sutures without artificial tube support regeneration across a 7-mm gap in the rat sciatic nerve. In the present study, the authors, compared this new approach with the use of autologous nerve grafts across short defects and examined whether the approach could be used to support regeneration across extended gaps and whether the interposition of a short nerve segment (the stepping-stone procedure) was applicable in this model. Methods. Longitudinal sutures were used to bridge 7- and 15-mm gaps in the rat sciatic nerve. Contralateral comparisons were made to nerve autografts in the 7-mm group and to sutures plus a short interposed nerve segment in the 15-mm group. Regeneration was evaluated at 2, 4, and 12 weeks by using immunocytochemical analysis for Schwann cells, neurofilament protein, and macrophages and at 12 weeks also by using histological examination, including morphometry in the distal tibial trunk and tetanic force measurements in the gastrocnemius muscle. Conclusions. The authors found that the results of regeneration after repair with longitudinal polyglactin sutures across short defects were not significantly different from those produced by the use of autologous nerve grafts. Regeneration, although poor, occurred along sutures across extended gaps and was significantly enhanced by an interposed nerve segment acting as a Schwann cell resource in this model.}},
author = {{Scherman, Peter and Lundborg, Göran and Kanje, Martin and Dahlin, Lars}},
issn = {{0022-3085}},
keywords = {{nerve regeneration; absorbable suture; nerve graft; interposed nerve segment; Schwann cell; muscle force; nerve fiber}},
language = {{eng}},
number = {{2}},
pages = {{316--323}},
publisher = {{American Association of Neurosurgeons}},
series = {{Journal of Neurosurgery}},
title = {{Neural regeneration along longitudinal polyglactin sutures across short and extended defects in the rat sciatic nerve}},
volume = {{95}},
year = {{2001}},
}