Infection with cagA- and vacA-positive and -negative strains of Helicobacter pylori in a mouse model
(2001) In Pathogens and Disease 30(2). p.115-120- Abstract
- To study the role of cytotoxin-associated protein (cagA) and vacuolating cytotoxin (vacA) in Helicobacter pylori infection in an experimental murine model, mice were infected with seven strains with different cagA and vacA status. Groups of 10 NMRI mice were challenged and were killed 5 weeks later. In a second study, 20 mice were challenged with a mixture of the same seven strains and killed 1, 3, 15 and 17 weeks post-inoculation. All seven strains were found to colonize the mice for the 5-week experimental period. Animals infected with vacA-positive strains, regardless of cagA status, showed an elevation of antibody titers. Two cagA-negative and vacA-positive strains and one cagA- and vacA-positive strain were found to 'take over' in the... (More)
- To study the role of cytotoxin-associated protein (cagA) and vacuolating cytotoxin (vacA) in Helicobacter pylori infection in an experimental murine model, mice were infected with seven strains with different cagA and vacA status. Groups of 10 NMRI mice were challenged and were killed 5 weeks later. In a second study, 20 mice were challenged with a mixture of the same seven strains and killed 1, 3, 15 and 17 weeks post-inoculation. All seven strains were found to colonize the mice for the 5-week experimental period. Animals infected with vacA-positive strains, regardless of cagA status, showed an elevation of antibody titers. Two cagA-negative and vacA-positive strains and one cagA- and vacA-positive strain were found to 'take over' in the mixed infection as analyzed by the randomly amplified polymorphic DNA-polymerase chain reaction technique and in one mouse stomach we found coexistence of two of the strains. We found no evidence of the different strains colonizing different parts of the stomach. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121018
- author
- Sturegård, Erik LU ; Sjunnesson, Håkan LU ; Nilsson, Hans-Olof LU ; Andersson, Rolf ; Areskoug, Christian and Wadström, Torkel LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Helicobacter pylori, Animal model, Mouse model, Cytotoxin-associated protein, Vacuolating cytotoxin, Randomly amplified polymorphic DNA-polymerase chain reaction
- in
- Pathogens and Disease
- volume
- 30
- issue
- 2
- pages
- 115 - 120
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:11267843
- scopus:0035074520
- ISSN
- 2049-632X
- DOI
- 10.1016/S0928-8244(00)00240-6
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Health Economics and Forensic Medicine (Closed 2012) (013040050), Clinical Microbiology, Malmö (013011000), Division of Medical Microbiology (013250400)
- id
- 86642274-987f-489b-bdc2-75102d7e5f04 (old id 1121018)
- date added to LUP
- 2016-04-01 12:01:38
- date last changed
- 2022-01-26 21:45:11
@article{86642274-987f-489b-bdc2-75102d7e5f04, abstract = {{To study the role of cytotoxin-associated protein (cagA) and vacuolating cytotoxin (vacA) in Helicobacter pylori infection in an experimental murine model, mice were infected with seven strains with different cagA and vacA status. Groups of 10 NMRI mice were challenged and were killed 5 weeks later. In a second study, 20 mice were challenged with a mixture of the same seven strains and killed 1, 3, 15 and 17 weeks post-inoculation. All seven strains were found to colonize the mice for the 5-week experimental period. Animals infected with vacA-positive strains, regardless of cagA status, showed an elevation of antibody titers. Two cagA-negative and vacA-positive strains and one cagA- and vacA-positive strain were found to 'take over' in the mixed infection as analyzed by the randomly amplified polymorphic DNA-polymerase chain reaction technique and in one mouse stomach we found coexistence of two of the strains. We found no evidence of the different strains colonizing different parts of the stomach.}}, author = {{Sturegård, Erik and Sjunnesson, Håkan and Nilsson, Hans-Olof and Andersson, Rolf and Areskoug, Christian and Wadström, Torkel}}, issn = {{2049-632X}}, keywords = {{Helicobacter pylori; Animal model; Mouse model; Cytotoxin-associated protein; Vacuolating cytotoxin; Randomly amplified polymorphic DNA-polymerase chain reaction}}, language = {{eng}}, number = {{2}}, pages = {{115--120}}, publisher = {{Wiley-Blackwell}}, series = {{Pathogens and Disease}}, title = {{Infection with cagA- and vacA-positive and -negative strains of Helicobacter pylori in a mouse model}}, url = {{http://dx.doi.org/10.1016/S0928-8244(00)00240-6}}, doi = {{10.1016/S0928-8244(00)00240-6}}, volume = {{30}}, year = {{2001}}, }