Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo
(2001) In FEMS Microbiology Letters 194(1). p.105-110- Abstract
- The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is... (More)
- The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121121
- author
- Kadioglu, Aras ; Sharpe, Jason A. ; Lazou, Irene ; Svanborg, Catharina LU ; Ockleford, Colin ; Mitchell, Tim J. and Andrew, Peter W.
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEMS Microbiology Letters
- volume
- 194
- issue
- 1
- pages
- 105 - 110
- publisher
- Oxford University Press
- external identifiers
-
- pmid:11150674
- scopus:0035163986
- ISSN
- 1574-6968
- DOI
- 10.1016/S0378-1097(00)00497-3
- language
- English
- LU publication?
- yes
- id
- 588813cc-33c6-4504-936c-8f815129b831 (old id 1121121)
- date added to LUP
- 2016-04-01 15:59:49
- date last changed
- 2022-04-22 18:54:00
@article{588813cc-33c6-4504-936c-8f815129b831,
abstract = {{The pneumococcus is the principle cause of bacterial pneumonia and also a major cause of bacterial meningitis. The mechanisms and sites of pneumococcal adherence and invasion of the respiratory tract in vivo are not clear however. We have made pneumococci expressing green fluorescent protein (GFP) and used it to trace pneumococcal adherence and invasion in vivo. By using GFP pneumococci we have shown bacterial adherence and invasion of broncho-epithelial cells in vivo by 4 h post-infection, with increases in pneumococcal invasiveness by 24 h. Using confocal image analysis we have shown varying levels of pneumococcal penetration and internalisation into host cells, as well as translocation through epithelial layers. To our knowledge this is the first report of pneumococcal invasion and cellular translocation in vivo.}},
author = {{Kadioglu, Aras and Sharpe, Jason A. and Lazou, Irene and Svanborg, Catharina and Ockleford, Colin and Mitchell, Tim J. and Andrew, Peter W.}},
issn = {{1574-6968}},
language = {{eng}},
number = {{1}},
pages = {{105--110}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Letters}},
title = {{Use of green fluorescent protein in visualisation of pneumococcal invasion of broncho-epithelial cells in vivo}},
url = {{http://dx.doi.org/10.1016/S0378-1097(00)00497-3}},
doi = {{10.1016/S0378-1097(00)00497-3}},
volume = {{194}},
year = {{2001}},
}