Dedifferentiation of serous ovarian cancer from cystic to solid tumors is associated with increased expression of mRNA for urokinase plasminogen activator (uPA), its receptor (uPAR) and its inhibitor (PAI-1)
Borgfeldt, Christer LU ; Hansson, Stefan LU
; Gustavsson, Barbro
LU
; Måsbäck, Anna
LU
and Casslén, Bertil
LU
(2001)
In International Journal of Cancer
92(4).
p.497-502
- Abstract
- The plasminogen activating system is involved in tumor growth and metastasis by degradation of extracellular matrix, and modulation of cell adhesion and migration. Benign and well-differentiated malignant ovarian tumors present as cystic lesions with preserved glandular morphology, whereas poorly differentiated tumors and metastases are solid with characteristic absence of glandular morphology. We analyzed the mRNAs for urokinase plasminogen activator (uPA), its receptor (uPAR), and inhibitor (PAI-1) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts. The mRNA expressing cells in the in situ hybridization sections were evaluated and counted by two different... (More)
- The plasminogen activating system is involved in tumor growth and metastasis by degradation of extracellular matrix, and modulation of cell adhesion and migration. Benign and well-differentiated malignant ovarian tumors present as cystic lesions with preserved glandular morphology, whereas poorly differentiated tumors and metastases are solid with characteristic absence of glandular morphology. We analyzed the mRNAs for urokinase plasminogen activator (uPA), its receptor (uPAR), and inhibitor (PAI-1) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts. The mRNA expressing cells in the in situ hybridization sections were evaluated and counted by two different observers. The number of mRNA expressing cells for uPA, uPAR and PAI-1 were all significantly increased in solid as compared with cystic malignant tumors. The increased expression of all three mRNA species was mainly located in the stroma of poorly differentiated tumors and metastases. Apart from being expressed in the stroma of these tumors, uPAR mRNA was also expressed by tumor cells located along the stromal/epithelial boarder. In addition, the tumor tissue content of uPA, uPAR and PAI-1 mRNAs as measured by Northern blots were higher in the solid as compared with the cystic tumors. Increased expression of uPA, uPAR and PAI-1 genes in the solid tumors suggest a correlation with a more aggressive phenotype. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1121182
- author
- Borgfeldt, Christer
LU
; Hansson, Stefan
LU
; Gustavsson, Barbro
LU
; Måsbäck, Anna
LU
and Casslén, Bertil
LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- human, ovarian neoplasm, mRNA analysis, urokinase, plasminogen activation
- in
- International Journal of Cancer
- volume
- 92
- issue
- 4
- pages
- 497 - 502
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:11304683
- scopus:0035874046
- ISSN
- 0020-7136
- DOI
- 10.1002/ijc.1215
- language
- English
- LU publication?
- yes
- id
- 4191771b-78b2-4788-9883-1493f5668704 (old id 1121182)
- date added to LUP
- 2016-04-01 12:23:02
- date last changed
- 2022-09-15 12:35:43
@article{4191771b-78b2-4788-9883-1493f5668704,
abstract = {{The plasminogen activating system is involved in tumor growth and metastasis by degradation of extracellular matrix, and modulation of cell adhesion and migration. Benign and well-differentiated malignant ovarian tumors present as cystic lesions with preserved glandular morphology, whereas poorly differentiated tumors and metastases are solid with characteristic absence of glandular morphology. We analyzed the mRNAs for urokinase plasminogen activator (uPA), its receptor (uPAR), and inhibitor (PAI-1) in serous ovarian tumors by in situ hybridization and by densitometric scanning of Northern blots prepared from tissue extracts. The mRNA expressing cells in the in situ hybridization sections were evaluated and counted by two different observers. The number of mRNA expressing cells for uPA, uPAR and PAI-1 were all significantly increased in solid as compared with cystic malignant tumors. The increased expression of all three mRNA species was mainly located in the stroma of poorly differentiated tumors and metastases. Apart from being expressed in the stroma of these tumors, uPAR mRNA was also expressed by tumor cells located along the stromal/epithelial boarder. In addition, the tumor tissue content of uPA, uPAR and PAI-1 mRNAs as measured by Northern blots were higher in the solid as compared with the cystic tumors. Increased expression of uPA, uPAR and PAI-1 genes in the solid tumors suggest a correlation with a more aggressive phenotype.}},
author = {{Borgfeldt, Christer and Hansson, Stefan and Gustavsson, Barbro and Måsbäck, Anna and Casslén, Bertil}},
issn = {{0020-7136}},
keywords = {{human; ovarian neoplasm; mRNA analysis; urokinase; plasminogen activation}},
language = {{eng}},
number = {{4}},
pages = {{497--502}},
publisher = {{John Wiley & Sons Inc.}},
series = {{International Journal of Cancer}},
title = {{Dedifferentiation of serous ovarian cancer from cystic to solid tumors is associated with increased expression of mRNA for urokinase plasminogen activator (uPA), its receptor (uPAR) and its inhibitor (PAI-1)}},
url = {{http://dx.doi.org/10.1002/ijc.1215}},
doi = {{10.1002/ijc.1215}},
volume = {{92}},
year = {{2001}},
}