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Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats

Kaastad, T S ; Reikeras, O ; Halvorsen, V ; Falch, J A ; Obrant, Karl LU and Nordsletten, L (2001) In Calcified Tissue International 69(2). p.102-108
Abstract
Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P < 0.005). Tibial and femoral weights and lengths showed no differences.... (More)
Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P < 0.005). Tibial and femoral weights and lengths showed no differences. Distal tibial trabecular bone volume was reduced in both ovx groups compare with sham (P < 0.005). Bone mineral density (BMD) was higher in sham than in Sham-D and both ovx groups (P < 0.005). Femoral area moment of inertia increased and ultimate stress decreased in Ovx-D compared with ovx (P < 0.05). Other biomechanical properties of the femoral shafts did not differ significantly. The femoral neck was significantly weaker in Ovx-D than in the other groups. In conclusion, ovx decreased tibial trabecular bone volume and both ovx and vit D depletion reduced femoral BMD in rats. Vit D depletion reduced the ultimate stress in the femoral shaft, and the combined depletion of estrogen and vit D significantly reduced the fracture strength in the femoral neck. This fits well with clinical evidence of how postmenopausal status combined with vit D deficiency lead to an increased risk of hip fractures, making this animal model a possible tool for investigating measures to prevent such fractures. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Calcified Tissue International
volume
69
issue
2
pages
102 - 108
publisher
Springer
external identifiers
  • pmid:11683422
  • scopus:0034887490
ISSN
1432-0827
DOI
10.1007/s00223-001-0009-2
language
English
LU publication?
yes
id
eef03470-2d5a-4693-a4a9-81851a569fb7 (old id 1121547)
date added to LUP
2016-04-01 15:25:16
date last changed
2022-01-28 05:16:10
@article{eef03470-2d5a-4693-a4a9-81851a569fb7,
  abstract     = {{Vitamin D (vit D) deficiency is common in the elderly, and the aim of this study was to investigate whether vit D deprivation in ovariectomized (ovx) and normal rats would reduce fracture strength. Forty mature female Wistar rats were randomized into four groups: two were ovariectomized (ovx) and two were sham-operated (sham). One ovx and one sham group were fed a vit D-deficient diet (Ovx-D and Sham-D), and the control groups were fed normal rat chow (Ovx and Sham) for 12 weeks. Vit D deficiency was substantiated after 12 weeks by undetectable serum concentrations of 25OHD in the Sham-D and Ovx-D groups. 85Sr activity was lower in Sham-D than in the other groups (P &lt; 0.005). Tibial and femoral weights and lengths showed no differences. Distal tibial trabecular bone volume was reduced in both ovx groups compare with sham (P &lt; 0.005). Bone mineral density (BMD) was higher in sham than in Sham-D and both ovx groups (P &lt; 0.005). Femoral area moment of inertia increased and ultimate stress decreased in Ovx-D compared with ovx (P &lt; 0.05). Other biomechanical properties of the femoral shafts did not differ significantly. The femoral neck was significantly weaker in Ovx-D than in the other groups. In conclusion, ovx decreased tibial trabecular bone volume and both ovx and vit D depletion reduced femoral BMD in rats. Vit D depletion reduced the ultimate stress in the femoral shaft, and the combined depletion of estrogen and vit D significantly reduced the fracture strength in the femoral neck. This fits well with clinical evidence of how postmenopausal status combined with vit D deficiency lead to an increased risk of hip fractures, making this animal model a possible tool for investigating measures to prevent such fractures.}},
  author       = {{Kaastad, T S and Reikeras, O and Halvorsen, V and Falch, J A and Obrant, Karl and Nordsletten, L}},
  issn         = {{1432-0827}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{102--108}},
  publisher    = {{Springer}},
  series       = {{Calcified Tissue International}},
  title        = {{Vitamin D deficiency and ovariectomy reduced the strength of the femoral neck in rats}},
  url          = {{http://dx.doi.org/10.1007/s00223-001-0009-2}},
  doi          = {{10.1007/s00223-001-0009-2}},
  volume       = {{69}},
  year         = {{2001}},
}