Conductance of GABAA channels activated by pentobarbitone in hippocampal neurons from newborn rats
(2003) In Journal of Physiology 552(1). p.13-22- Abstract
- Neurons were obtained from the CA1 region of the hippocampus of newborn rats and maintained in culture. Channels were activated by pentobarbitone in cell-attached, inside-out or outside-out patches, normally by applying pentobarbitone in flowing bath solution. Currents were outwardly rectifying and blocked by bicuculline, properties of GABAA channels in these cells. Maximum channel conductance increased as pentobarbitone concentration was increased to 500 microM but conductance then decreased as pentobarbitone concentration was raised further. The best fit of a Hill-type equation to the relationship between maximum channel conductance and pentobarbitone concentration (up to 500 microM) gave an EC50 of 41 microM, a maximum conductance of 36... (More)
- Neurons were obtained from the CA1 region of the hippocampus of newborn rats and maintained in culture. Channels were activated by pentobarbitone in cell-attached, inside-out or outside-out patches, normally by applying pentobarbitone in flowing bath solution. Currents were outwardly rectifying and blocked by bicuculline, properties of GABAA channels in these cells. Maximum channel conductance increased as pentobarbitone concentration was increased to 500 microM but conductance then decreased as pentobarbitone concentration was raised further. The best fit of a Hill-type equation to the relationship between maximum channel conductance and pentobarbitone concentration (up to 500 microM) gave an EC50 of 41 microM, a maximum conductance of 36 pS and a Hill coefficient of 1.6. Bicuculline decreased the maximum conductance of the channels activated by pentobarbitone, with an IC50 of 224 microM. Diazepam increased channel conductance, with a maximum effect being obtained with 1 microM diazepam. Diazepam (1 microM) decreased the EC50 of the pentobarbitone effect on channel conductance from 41 microM to 7.2 microM and increased maximum conductance to 72 pS. We conclude that GABAA channel conductance is related to the concentration of the allosteric agonist pentobarbitone. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1126890
- author
- Eghbali, Mansoureh ; Birnir, Bryndis LU and Gage, Peter W
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Physiology
- volume
- 552
- issue
- 1
- pages
- 13 - 22
- publisher
- The Physiological Society
- external identifiers
-
- pmid:12897171
- scopus:0142125332
- ISSN
- 1469-7793
- DOI
- 10.1113/jphysiol.2003.047415
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: GABA Channels in Physiology and Pharmacology (013241570)
- id
- 6257bb2e-be45-4707-a092-df0c751b461c (old id 1126890)
- date added to LUP
- 2016-04-01 17:05:42
- date last changed
- 2022-04-15 17:11:07
@article{6257bb2e-be45-4707-a092-df0c751b461c,
abstract = {{Neurons were obtained from the CA1 region of the hippocampus of newborn rats and maintained in culture. Channels were activated by pentobarbitone in cell-attached, inside-out or outside-out patches, normally by applying pentobarbitone in flowing bath solution. Currents were outwardly rectifying and blocked by bicuculline, properties of GABAA channels in these cells. Maximum channel conductance increased as pentobarbitone concentration was increased to 500 microM but conductance then decreased as pentobarbitone concentration was raised further. The best fit of a Hill-type equation to the relationship between maximum channel conductance and pentobarbitone concentration (up to 500 microM) gave an EC50 of 41 microM, a maximum conductance of 36 pS and a Hill coefficient of 1.6. Bicuculline decreased the maximum conductance of the channels activated by pentobarbitone, with an IC50 of 224 microM. Diazepam increased channel conductance, with a maximum effect being obtained with 1 microM diazepam. Diazepam (1 microM) decreased the EC50 of the pentobarbitone effect on channel conductance from 41 microM to 7.2 microM and increased maximum conductance to 72 pS. We conclude that GABAA channel conductance is related to the concentration of the allosteric agonist pentobarbitone.}},
author = {{Eghbali, Mansoureh and Birnir, Bryndis and Gage, Peter W}},
issn = {{1469-7793}},
language = {{eng}},
number = {{1}},
pages = {{13--22}},
publisher = {{The Physiological Society}},
series = {{Journal of Physiology}},
title = {{Conductance of GABAA channels activated by pentobarbitone in hippocampal neurons from newborn rats}},
url = {{http://dx.doi.org/10.1113/jphysiol.2003.047415}},
doi = {{10.1113/jphysiol.2003.047415}},
volume = {{552}},
year = {{2003}},
}