SERF: a new antigen in the Cromer blood group system
(2004) In Transfusion Medicine 14(4). p.313-318- Abstract
- The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence... (More)
- The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence analysis revealed a substitution of 647C>T in exon 5 DAF in the proband. The proband's two children and two of 100 Thais were heterozygotes 647C/T. Analysis using DAF deletion mutants revealed the antigenic determinant to be within short consensus repeat 3 (SCR3), which is encoded by exon 5. This study describes a novel high incidence antigen (SERF) in the Cromer blood group system characterized by the amino acid proline at position 182 in SCR3 of DAF. The SERF-negative proband has a substitution mutation that predicts for leucine at this position. SERF has been provisionally assigned the International Society of Blood Transfusion number 021.012 (CROM 12). (Less)
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- author
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transfusion Medicine
- volume
- 14
- issue
- 4
- pages
- 313 - 318
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:15285728
- scopus:4043168377
- ISSN
- 0958-7578
- DOI
- 10.1111/j.0958-7578.2004.00519.x
- language
- English
- LU publication?
- no
- id
- 07fb3dee-c8c7-4f15-b4b2-279c0afeb55b (old id 1129236)
- date added to LUP
- 2016-04-01 16:49:46
- date last changed
- 2022-01-28 22:28:33
@article{07fb3dee-c8c7-4f15-b4b2-279c0afeb55b,
abstract = {{The Cromer blood group system consists of eight high incidence and three low incidence antigens carried on decay-accelerating factor (DAF). This report describes the identification and characterization of a new Cromer high incidence antigen, named SERF. Sequence analyses of DNA from a Thai female whose serum contained the alloantibody to a high incidence antigen in the Cromer blood group system (anti-SERF) and from her two children were performed. Reverse transcriptase-polymerase chain reaction (RT-PCR) and sequence analysis on cDNA from the proband and PCR-restriction fragment length polymorphism analysis on DNA from Thais were also performed. To map the epitope, DAF deletion mutants were tested by immunoblotting with anti-SERF. Sequence analysis revealed a substitution of 647C>T in exon 5 DAF in the proband. The proband's two children and two of 100 Thais were heterozygotes 647C/T. Analysis using DAF deletion mutants revealed the antigenic determinant to be within short consensus repeat 3 (SCR3), which is encoded by exon 5. This study describes a novel high incidence antigen (SERF) in the Cromer blood group system characterized by the amino acid proline at position 182 in SCR3 of DAF. The SERF-negative proband has a substitution mutation that predicts for leucine at this position. SERF has been provisionally assigned the International Society of Blood Transfusion number 021.012 (CROM 12).}},
author = {{Banks, J and Poole, J and Ahrens, N and Seltsam, A and Salama, A and Hue-Roye, K and Storry, Jill and Palacajornsuk, P and Ma, B-W and Lublin, D M and Reid, M E}},
issn = {{0958-7578}},
language = {{eng}},
number = {{4}},
pages = {{313--318}},
publisher = {{Wiley-Blackwell}},
series = {{Transfusion Medicine}},
title = {{SERF: a new antigen in the Cromer blood group system}},
url = {{http://dx.doi.org/10.1111/j.0958-7578.2004.00519.x}},
doi = {{10.1111/j.0958-7578.2004.00519.x}},
volume = {{14}},
year = {{2004}},
}