A new hybrid RHCE gene (CeNR) is responsible for expression of a novel antigen
(2004) In Transfusion 44(7). p.1047-1051- Abstract
- BACKGROUND: The red blood cells (RBCs) of a patient, known to have the probable DC(W)(e)/D-- phenotype, typed as D(W)- and Rh32- but were unexpectedly agglutinated by an anti-D(W)/Rh32 serum. The reactivity suggested that the RBCs carried a novel antigen and that the molecular background of this DC(W)(e)/D-- phenotype might be different from those reported. The purpose of this study was to determine the molecular basis of the Rh phenotype. STUDY DESIGN AND METHODS: Samples were obtained for family studies. Standard hemagglutination methods were used. RH mRNA transcripts were isolated by reverse transcription-polymerase chain reaction and sequenced. RESULTS: Sequence analysis revealed that the probond had three different RH transcripts: a... (More)
- BACKGROUND: The red blood cells (RBCs) of a patient, known to have the probable DC(W)(e)/D-- phenotype, typed as D(W)- and Rh32- but were unexpectedly agglutinated by an anti-D(W)/Rh32 serum. The reactivity suggested that the RBCs carried a novel antigen and that the molecular background of this DC(W)(e)/D-- phenotype might be different from those reported. The purpose of this study was to determine the molecular basis of the Rh phenotype. STUDY DESIGN AND METHODS: Samples were obtained for family studies. Standard hemagglutination methods were used. RH mRNA transcripts were isolated by reverse transcription-polymerase chain reaction and sequenced. RESULTS: Sequence analysis revealed that the probond had three different RH transcripts: a normal RHD and two different hybrid transcripts from the RHCE locus, a RHCE-D hybrid with exon 1 from RHCE associated with the D-- haplotype, and a new RHCE-D hybrid. In this new hybrid, exons 1 to 5 are RHCe-specific and exons 6 to 10 correspond to RHD. The C(W) antigen is also encoded by this hybrid gene. Family studies confirmed that the new RHCE-D hybrid is linked in cis to conventional RHD. CONCLUSION: A new RHCE-D structure is associated with altered expression of C and e antigens in this family and the generation of a novel low-prevalence antigen (CENR). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1129248
- author
- Westhoff, Connie M ; Storry, Jill LU ; Walker, Phyllis ; Lomas-Francis, Christine and Reid, Marion E
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transfusion
- volume
- 44
- issue
- 7
- pages
- 1047 - 1051
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:15225246
- scopus:3042594855
- ISSN
- 1537-2995
- DOI
- 10.1111/j.1537-2995.2004.04003.x
- language
- English
- LU publication?
- no
- id
- 4a106499-d455-4a97-817c-54e14878d2d7 (old id 1129248)
- date added to LUP
- 2016-04-01 15:25:50
- date last changed
- 2022-01-28 05:16:15
@article{4a106499-d455-4a97-817c-54e14878d2d7,
abstract = {{BACKGROUND: The red blood cells (RBCs) of a patient, known to have the probable DC(W)(e)/D-- phenotype, typed as D(W)- and Rh32- but were unexpectedly agglutinated by an anti-D(W)/Rh32 serum. The reactivity suggested that the RBCs carried a novel antigen and that the molecular background of this DC(W)(e)/D-- phenotype might be different from those reported. The purpose of this study was to determine the molecular basis of the Rh phenotype. STUDY DESIGN AND METHODS: Samples were obtained for family studies. Standard hemagglutination methods were used. RH mRNA transcripts were isolated by reverse transcription-polymerase chain reaction and sequenced. RESULTS: Sequence analysis revealed that the probond had three different RH transcripts: a normal RHD and two different hybrid transcripts from the RHCE locus, a RHCE-D hybrid with exon 1 from RHCE associated with the D-- haplotype, and a new RHCE-D hybrid. In this new hybrid, exons 1 to 5 are RHCe-specific and exons 6 to 10 correspond to RHD. The C(W) antigen is also encoded by this hybrid gene. Family studies confirmed that the new RHCE-D hybrid is linked in cis to conventional RHD. CONCLUSION: A new RHCE-D structure is associated with altered expression of C and e antigens in this family and the generation of a novel low-prevalence antigen (CENR).}},
author = {{Westhoff, Connie M and Storry, Jill and Walker, Phyllis and Lomas-Francis, Christine and Reid, Marion E}},
issn = {{1537-2995}},
language = {{eng}},
number = {{7}},
pages = {{1047--1051}},
publisher = {{Wiley-Blackwell}},
series = {{Transfusion}},
title = {{A new hybrid RHCE gene (CeNR) is responsible for expression of a novel antigen}},
url = {{http://dx.doi.org/10.1111/j.1537-2995.2004.04003.x}},
doi = {{10.1111/j.1537-2995.2004.04003.x}},
volume = {{44}},
year = {{2004}},
}