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High throughput genotyping of oncogenic human papilloma viruses using MALDI-TOF mass spectrometry.

Söderlund Strand, Anna LU ; Dillner, Joakim LU and Carlson, Joyce LU (2008) In Clinical Chemistry 54(1). p.86-92
Abstract
Background: Human papilloma virus (HPV) is the major cause of cervical cancer. Use of HPV genotyping in cervical screening programs and for monitoring the effectiveness of HPV vaccination programs requires access to economical, high-throughput technology.



Methods: We used the Sequenom MassARRAY platform to develop a high-throughput mass spectrometric (MS) method for detecting 14 specific oncogenic HPV genotypes in multiplex PCR products. We compared results from 532 cervical cell samples to the comparison method, reverse dot blot hybridization (RDBH).



Results: The MS method detected all samples found positive by RDBH. In addition, the MS method identified 5 cases of cervical disease (cervical... (More)
Background: Human papilloma virus (HPV) is the major cause of cervical cancer. Use of HPV genotyping in cervical screening programs and for monitoring the effectiveness of HPV vaccination programs requires access to economical, high-throughput technology.



Methods: We used the Sequenom MassARRAY platform to develop a high-throughput mass spectrometric (MS) method for detecting 14 specific oncogenic HPV genotypes in multiplex PCR products. We compared results from 532 cervical cell samples to the comparison method, reverse dot blot hybridization (RDBH).



Results: The MS method detected all samples found positive by RDBH. In addition, the MS method identified 5 cases of cervical disease (cervical intraepithelial neoplasia of grade I or higher) that RDBH analysis had missed. Discrepancies in specific genotypes were noted in 20 samples, all positive by MS, with an overall concordance of {kappa} = 0.945.



Conclusions: The MS high-throughput method, with a processing capacity of 10 x 384 samples within 2 working days and at a consumables cost of about US$2 per sample, performed as well as or better than the comparison method. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Chemistry
volume
54
issue
1
pages
86 - 92
publisher
American Association for Clinical Chemistry
external identifiers
  • wos:000252295600015
  • scopus:38749100192
ISSN
0009-9147
DOI
10.1373/clinchem.2007.092627
language
English
LU publication?
yes
id
7feccf54-d43d-45af-87a5-797057991101 (old id 1142932)
date added to LUP
2016-04-01 12:23:02
date last changed
2022-01-27 02:59:48
@article{7feccf54-d43d-45af-87a5-797057991101,
  abstract     = {{Background: Human papilloma virus (HPV) is the major cause of cervical cancer. Use of HPV genotyping in cervical screening programs and for monitoring the effectiveness of HPV vaccination programs requires access to economical, high-throughput technology.<br/><br>
<br/><br>
Methods: We used the Sequenom MassARRAY platform to develop a high-throughput mass spectrometric (MS) method for detecting 14 specific oncogenic HPV genotypes in multiplex PCR products. We compared results from 532 cervical cell samples to the comparison method, reverse dot blot hybridization (RDBH).<br/><br>
<br/><br>
Results: The MS method detected all samples found positive by RDBH. In addition, the MS method identified 5 cases of cervical disease (cervical intraepithelial neoplasia of grade I or higher) that RDBH analysis had missed. Discrepancies in specific genotypes were noted in 20 samples, all positive by MS, with an overall concordance of {kappa} = 0.945.<br/><br>
<br/><br>
Conclusions: The MS high-throughput method, with a processing capacity of 10 x 384 samples within 2 working days and at a consumables cost of about US$2 per sample, performed as well as or better than the comparison method.}},
  author       = {{Söderlund Strand, Anna and Dillner, Joakim and Carlson, Joyce}},
  issn         = {{0009-9147}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{86--92}},
  publisher    = {{American Association for Clinical Chemistry}},
  series       = {{Clinical Chemistry}},
  title        = {{High throughput genotyping of oncogenic human papilloma viruses using MALDI-TOF mass spectrometry.}},
  url          = {{http://dx.doi.org/10.1373/clinchem.2007.092627}},
  doi          = {{10.1373/clinchem.2007.092627}},
  volume       = {{54}},
  year         = {{2008}},
}