Different cell cycle kinetic effects of N1,N11-diethylnorspermine-induced polyamine depletion in four human breast cancer cell lines.
Myhre, Louise LU ; Alm, Kersti LU ; Hegardt, Cecilia LU ; Staaf, Johan LU
; Jönsson, Göran B
LU
; Larsson, Sara
LU
and Oredsson, Stina
LU
(2008)
In Anti-Cancer Drugs
19(4).
p.359-368
- Abstract
- Polyamine analogues are presently undergoing clinical evaluation in the treatment of cancer. To better understand under what circumstances treatment with a polyamine analogue will yield beneficial results, we have investigated the effect of N,N-diethylnorspermine (DENSPM) on cell cycle kinetics of the human breast cancer cell lines SK-BR-3, MCF-7, HCC1937, and L56Br-C1. A bromodeoxyuridine-DNA flow cytometry method was used to evaluate the treatment with 10 micromol/l DENSPM on cell cycle kinetics. A correlation between polyamine pool size after DENSPM treatment and cell cycle kinetic effects was found. The most sensitive cell cycle phase was the S phase, followed by an effect on the G2+M phase and then the G1/S transition. The levels of a... (More)
- Polyamine analogues are presently undergoing clinical evaluation in the treatment of cancer. To better understand under what circumstances treatment with a polyamine analogue will yield beneficial results, we have investigated the effect of N,N-diethylnorspermine (DENSPM) on cell cycle kinetics of the human breast cancer cell lines SK-BR-3, MCF-7, HCC1937, and L56Br-C1. A bromodeoxyuridine-DNA flow cytometry method was used to evaluate the treatment with 10 micromol/l DENSPM on cell cycle kinetics. A correlation between polyamine pool size after DENSPM treatment and cell cycle kinetic effects was found. The most sensitive cell cycle phase was the S phase, followed by an effect on the G2+M phase and then the G1/S transition. The levels of a number of cell cycle regulatory proteins such as cyclin E1, cyclin A2, and cyclin B1 were lowered by DENSPM treatment, which may explain the effects on cell cycle kinetics. The two cell lines that were most sensitive to DENSPM treatment belong to the basal-like subtype of breast cancer and they were deficient with respect to p53, BRCA1, and RB1. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1154588
- author
- Myhre, Louise
LU
; Alm, Kersti
LU
; Hegardt, Cecilia
LU
; Staaf, Johan
LU
; Jönsson, Göran B
LU
; Larsson, Sara
LU
and Oredsson, Stina
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Anti-Cancer Drugs
- volume
- 19
- issue
- 4
- pages
- 359 - 368
- publisher
- Rapid Communications
- external identifiers
-
- wos:000254247000004
- pmid:18454046
- scopus:47949093670
- pmid:18454046
- ISSN
- 0959-4973
- DOI
- 10.1097/CAD.0b013e3282f7f518
- language
- English
- LU publication?
- yes
- id
- 3485bb93-05ae-4570-9523-e00b49cb3c0c (old id 1154588)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18454046?dopt=Abstract
- date added to LUP
- 2016-04-04 08:29:36
- date last changed
- 2022-01-29 03:26:51
@article{3485bb93-05ae-4570-9523-e00b49cb3c0c,
abstract = {{Polyamine analogues are presently undergoing clinical evaluation in the treatment of cancer. To better understand under what circumstances treatment with a polyamine analogue will yield beneficial results, we have investigated the effect of N,N-diethylnorspermine (DENSPM) on cell cycle kinetics of the human breast cancer cell lines SK-BR-3, MCF-7, HCC1937, and L56Br-C1. A bromodeoxyuridine-DNA flow cytometry method was used to evaluate the treatment with 10 micromol/l DENSPM on cell cycle kinetics. A correlation between polyamine pool size after DENSPM treatment and cell cycle kinetic effects was found. The most sensitive cell cycle phase was the S phase, followed by an effect on the G2+M phase and then the G1/S transition. The levels of a number of cell cycle regulatory proteins such as cyclin E1, cyclin A2, and cyclin B1 were lowered by DENSPM treatment, which may explain the effects on cell cycle kinetics. The two cell lines that were most sensitive to DENSPM treatment belong to the basal-like subtype of breast cancer and they were deficient with respect to p53, BRCA1, and RB1.}},
author = {{Myhre, Louise and Alm, Kersti and Hegardt, Cecilia and Staaf, Johan and Jönsson, Göran B and Larsson, Sara and Oredsson, Stina}},
issn = {{0959-4973}},
language = {{eng}},
number = {{4}},
pages = {{359--368}},
publisher = {{Rapid Communications}},
series = {{Anti-Cancer Drugs}},
title = {{Different cell cycle kinetic effects of N1,N11-diethylnorspermine-induced polyamine depletion in four human breast cancer cell lines.}},
url = {{http://dx.doi.org/10.1097/CAD.0b013e3282f7f518}},
doi = {{10.1097/CAD.0b013e3282f7f518}},
volume = {{19}},
year = {{2008}},
}