Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters
(2008) In Bioprocess and Biosystems Engineering 31(4). p.369-377- Abstract
- A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration > 0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate > 0 g/L.h was found. Strategies for improving the co-substrate utilization by genetic engineering... (More)
- A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration > 0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate > 0 g/L.h was found. Strategies for improving the co-substrate utilization by genetic engineering of the transport systems are furthermore suggested based on simulations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1203529
- author
- Wiman, Magnus LU ; Andersson, Jonas and Lidén, Gunnar LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cerevisiae, Saccharomyces, xylose uptake, jinetic modeling, hexose transporters
- in
- Bioprocess and Biosystems Engineering
- volume
- 31
- issue
- 4
- pages
- 369 - 377
- publisher
- Springer
- external identifiers
-
- wos:000255998300012
- scopus:44149113738
- pmid:17985160
- ISSN
- 1615-7605
- DOI
- 10.1007/s00449-007-0169-1
- language
- English
- LU publication?
- yes
- id
- b058c0d3-c7ff-4c85-b559-f1853b58b13d (old id 1203529)
- date added to LUP
- 2016-04-01 13:08:16
- date last changed
- 2023-12-11 10:35:44
@article{b058c0d3-c7ff-4c85-b559-f1853b58b13d,
abstract = {{A kinetic model for glucose and xylose co-substrate uptake in Saccharomyces cerevisiae is presented. The model couples the enzyme kinetics with the glucose-dependent genetic expression of the individual transport proteins. This novel approach implies several options for optimizing the co-substrate utilization. Interestingly, the simulations predict a maximum xylose uptake rate at a glucose concentration > 0 g/L, which suggests that the genetic expressions of the considered transport proteins are of importance when optimizing the xylose uptake. This was also evident in fed-batch simulations, where a distinct optimal glucose addition rate > 0 g/L.h was found. Strategies for improving the co-substrate utilization by genetic engineering of the transport systems are furthermore suggested based on simulations.}},
author = {{Wiman, Magnus and Andersson, Jonas and Lidén, Gunnar}},
issn = {{1615-7605}},
keywords = {{cerevisiae; Saccharomyces; xylose uptake; jinetic modeling; hexose transporters}},
language = {{eng}},
number = {{4}},
pages = {{369--377}},
publisher = {{Springer}},
series = {{Bioprocess and Biosystems Engineering}},
title = {{Modeling simultaneous glucose and xylose uptake in Saccharomyces cerevisiae from kinetics and gene expression of sugar transporters}},
url = {{http://dx.doi.org/10.1007/s00449-007-0169-1}},
doi = {{10.1007/s00449-007-0169-1}},
volume = {{31}},
year = {{2008}},
}