Effect of polyamine deficiency on proteins involved in Okazaki fragment maturation.
Johansson, Veronica LU ; Falck Miniotis, Maria ; Hegardt, Cecilia LU ; Jönsson, Göran B LU ; Staaf, Johan LU
; Berntsson, Pia
LU
; Oredsson, Stina
LU
and Alm, Kersti
LU
(2008)
In Cell Biology International
32.
p.1467-1477
- Abstract
- Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion... (More)
- Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion sensitivity, something that can be useful for cancer treatment with polyamine analogues. DENSPM treatment affects the cellular distribution of FEN1 in L56Br-C1 cells, but not in MCF-7 cells, implying that FEN1 is affected by or involved in DENSPM-induced apoptosis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1243097
- author
- Johansson, Veronica
LU
; Falck Miniotis, Maria
; Hegardt, Cecilia
LU
; Jönsson, Göran B
LU
; Staaf, Johan
LU
; Berntsson, Pia
LU
; Oredsson, Stina
LU
and Alm, Kersti
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CGP 48664, DENSPM, human breast cancer, FEN1, DNA ligase I
- in
- Cell Biology International
- volume
- 32
- pages
- 1467 - 1477
- publisher
- Elsevier
- external identifiers
-
- wos:000261001100001
- scopus:55949114642
- ISSN
- 1095-8355
- DOI
- 10.1016/j.cellbi.2008.08.018
- language
- English
- LU publication?
- yes
- id
- 0daf9ffa-f3ad-4d3b-a803-9e10fb76def5 (old id 1243097)
- date added to LUP
- 2016-04-01 12:25:25
- date last changed
- 2022-03-21 03:56:00
@article{0daf9ffa-f3ad-4d3b-a803-9e10fb76def5,
abstract = {{Polyamine depletion causes S phase prolongation, and earlier studies indicate that the elongation step of DNA replication is affected. This led us to investigate the effects of polyamine depletion on enzymes crucial for Okazaki fragment maturation in the two breast cancer cell lines MCF-7 and L56Br-C1. In MCF-7 cells, treatment with N(1),N(11)-diethylnorspermine (DENSPM) causes S phase prolongation. In L56Br-C1 cells the prolongation is followed by massive apoptosis. In the present study we show that L56Br-C1 cells have substantially lower basal expressions of two Okazaki fragment maturation key proteins, DNA ligase I and FEN1, than MCF-7 cells. Thus, these two proteins might be promising markers for prediction of polyamine depletion sensitivity, something that can be useful for cancer treatment with polyamine analogues. DENSPM treatment affects the cellular distribution of FEN1 in L56Br-C1 cells, but not in MCF-7 cells, implying that FEN1 is affected by or involved in DENSPM-induced apoptosis.}},
author = {{Johansson, Veronica and Falck Miniotis, Maria and Hegardt, Cecilia and Jönsson, Göran B and Staaf, Johan and Berntsson, Pia and Oredsson, Stina and Alm, Kersti}},
issn = {{1095-8355}},
keywords = {{CGP 48664; DENSPM; human breast cancer; FEN1; DNA ligase I}},
language = {{eng}},
pages = {{1467--1477}},
publisher = {{Elsevier}},
series = {{Cell Biology International}},
title = {{Effect of polyamine deficiency on proteins involved in Okazaki fragment maturation.}},
url = {{http://dx.doi.org/10.1016/j.cellbi.2008.08.018}},
doi = {{10.1016/j.cellbi.2008.08.018}},
volume = {{32}},
year = {{2008}},
}