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Affinity Two-Phase Partitioning in Acoustically Levitated Drops

Santesson, Sabina LU ; Barinaga-Rementeria Ramírez, Irene LU ; Viberg, Peter LU ; Jergil, Bengt LU and Nilsson, Staffan LU (2004) In Analytical Chemistry 76(2). p.303-308
Abstract
Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by... (More)
Miniaturized (<1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by readjusting the field. NeutrAvidin-dextran brought about the redistribution of biotinylated liposomes from the poly(ethylene glycol)-rich phase into the dextran-rich phase. Thus, an entire affinity two-phase separation procedure, including mixing of the phases and incubation to allow affinity interactions to develop under constant volume, followed by phase separation under controlled evaporation, can be performed in a single levitated drop. This miniaturized technique would allow the separation of biologically active membranes or organelles from individual cells for analysis. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
76
issue
2
pages
303 - 308
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:14719875
  • wos:000188198800008
  • scopus:0346459923
  • pmid:14719875
ISSN
1520-6882
DOI
10.1021/ac034951h
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biochemistry and Structural Biology (S) (000006142)
id
a6b42434-befc-4044-8056-3158dc1c3420 (old id 126105)
date added to LUP
2016-04-04 12:10:45
date last changed
2022-03-16 00:24:42
@article{a6b42434-befc-4044-8056-3158dc1c3420,
  abstract     = {{Miniaturized (&lt;1 L) biospecific affinity two-phase partitioning in an acoustically levitated drop is described. Miniaturization commonly gives unfavorable surface/volume ratios, but in the levitation approach adsorption problems are minimized since the only surrounding wall is the liquid/air interface of the drop. Biotinylated liposomes were partitioned in aqueous poly(ethylene glycol)/dextran two-phase drops with NeutrAvidin-dextran as the affinity ligand. A two-phase drop was trapped and manipulated in a node of a standing ultrasonic wave. Alternatively, a two-phase system was formed by levitation and evaporation of a polymer one-phase drop. Phase mixing was achieved by adjusting the ultrasonic field and phase separation by readjusting the field. NeutrAvidin-dextran brought about the redistribution of biotinylated liposomes from the poly(ethylene glycol)-rich phase into the dextran-rich phase. Thus, an entire affinity two-phase separation procedure, including mixing of the phases and incubation to allow affinity interactions to develop under constant volume, followed by phase separation under controlled evaporation, can be performed in a single levitated drop. This miniaturized technique would allow the separation of biologically active membranes or organelles from individual cells for analysis.}},
  author       = {{Santesson, Sabina and Barinaga-Rementeria Ramírez, Irene and Viberg, Peter and Jergil, Bengt and Nilsson, Staffan}},
  issn         = {{1520-6882}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{303--308}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Analytical Chemistry}},
  title        = {{Affinity Two-Phase Partitioning in Acoustically Levitated Drops}},
  url          = {{http://dx.doi.org/10.1021/ac034951h}},
  doi          = {{10.1021/ac034951h}},
  volume       = {{76}},
  year         = {{2004}},
}