Development of biosensors based on hexacyanoferrates.
(2000) In Talanta 52(5). p.791-799- Abstract
- Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were electrodeposited on glassy carbon electrodes providing a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Additionally glucose oxidase was immobilized on top of these electrodes to form glucose biosensors. The biosensors were made by casting glucose oxidase-Nafion layers onto the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution (5 muM H(2)O(2) for PB, 5 mM H(2)O(2) for CuHCF and 2.5 mM glucose for both) over 5-10 h in a flow-injection system with the electrodes polarized at -50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose... (More)
- Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were electrodeposited on glassy carbon electrodes providing a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Additionally glucose oxidase was immobilized on top of these electrodes to form glucose biosensors. The biosensors were made by casting glucose oxidase-Nafion layers onto the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution (5 muM H(2)O(2) for PB, 5 mM H(2)O(2) for CuHCF and 2.5 mM glucose for both) over 5-10 h in a flow-injection system with the electrodes polarized at -50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose biosensors demonstrated suitability for glucose determination: 0.0-2.5 mM (R(2)=0.9977) for PB and 0.0-10 mM (R(2)=0.9927) for CuHCF, respectively. The visualization of the redox catalyst modifiers (PB and CuHCF films) was presented by scanning electron micrographs. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1261754
- author
- de Mattos, I L ; Gorton, Lo LU ; Laurell, Thomas LU ; Malinauskas, A and Karyakin, A A
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Talanta
- volume
- 52
- issue
- 5
- pages
- 791 - 799
- publisher
- Elsevier
- external identifiers
-
- pmid:18968038
- scopus:0034675042
- ISSN
- 1873-3573
- DOI
- 10.1016/S0039-9140(00)00409-4
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)
- id
- 964385b3-fa4a-4038-be54-2fe61bd38c91 (old id 1261754)
- date added to LUP
- 2016-04-01 15:59:34
- date last changed
- 2022-01-28 08:33:23
@article{964385b3-fa4a-4038-be54-2fe61bd38c91,
abstract = {{Ferric and copper hexacyanoferrates (PB and CuHCF, respectively) were electrodeposited on glassy carbon electrodes providing a suitable catalytic surface for the amperometric detection of hydrogen peroxide. Additionally glucose oxidase was immobilized on top of these electrodes to form glucose biosensors. The biosensors were made by casting glucose oxidase-Nafion layers onto the surface of the modified electrodes. The operational stability of the films and the biosensors were evaluated by injecting a standard solution (5 muM H(2)O(2) for PB, 5 mM H(2)O(2) for CuHCF and 2.5 mM glucose for both) over 5-10 h in a flow-injection system with the electrodes polarized at -50 (PB) and -200 mV (CuHCF) versus Ag/AgCl, respectively. The glucose biosensors demonstrated suitability for glucose determination: 0.0-2.5 mM (R(2)=0.9977) for PB and 0.0-10 mM (R(2)=0.9927) for CuHCF, respectively. The visualization of the redox catalyst modifiers (PB and CuHCF films) was presented by scanning electron micrographs.}},
author = {{de Mattos, I L and Gorton, Lo and Laurell, Thomas and Malinauskas, A and Karyakin, A A}},
issn = {{1873-3573}},
language = {{eng}},
number = {{5}},
pages = {{791--799}},
publisher = {{Elsevier}},
series = {{Talanta}},
title = {{Development of biosensors based on hexacyanoferrates.}},
url = {{http://dx.doi.org/10.1016/S0039-9140(00)00409-4}},
doi = {{10.1016/S0039-9140(00)00409-4}},
volume = {{52}},
year = {{2000}},
}