Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Positively Regulated Glycerol/G3P-Dependent Bacillus subtilis Gene Expression System Based on Anti-Termination.

Lewin, Anna LU ; Su, Xiao-Dong and Hederstedt, Lars LU (2009) In Journal of Molecular Microbiology and Biotechnology 17. p.61-70
Abstract
Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive... (More)
Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anti-termination, glp, Glycerol inducible, Bacillus subtilis
in
Journal of Molecular Microbiology and Biotechnology
volume
17
pages
61 - 70
publisher
Karger
external identifiers
  • wos:000268373100002
  • scopus:68149160868
  • pmid:18946204
ISSN
1464-1801
DOI
10.1159/000166210
language
English
LU publication?
yes
id
49b62a06-b757-4eec-8b8b-37961d8df1c1 (old id 1262036)
date added to LUP
2016-04-01 13:00:46
date last changed
2022-01-27 08:51:32
@article{49b62a06-b757-4eec-8b8b-37961d8df1c1,
  abstract     = {{Plasmid pLALA was constructed for glycerol or glycerol-3-phosphate inducible plasmid-borne gene expression in Bacillus subtilis and closely related Gram-positive bacteria. Gene expression using pLALA is based on anti-termination of transcription and involves the B. subtilis GlpP protein that in the presence of glycerol-3-phosphate acts as an anti-terminator protein by binding to the 5'-untranslated region of glpD mRNA. Properties and the usefulness of the system, denoted LALA, were validated by inducible production in B. subtilis strainsof two water-soluble proteins (beta-galactosidase and a protein phospho-tyrosine phosphatase), and one integral membrane protein (heme A synthase). Advantages with LALA is that it is based on positive control, does not involve a DNA-binding protein, and that glycerol, a cheap and stable compound, can be used as inducer of gene expression.}},
  author       = {{Lewin, Anna and Su, Xiao-Dong and Hederstedt, Lars}},
  issn         = {{1464-1801}},
  keywords     = {{Anti-termination; glp; Glycerol inducible; Bacillus subtilis}},
  language     = {{eng}},
  pages        = {{61--70}},
  publisher    = {{Karger}},
  series       = {{Journal of Molecular Microbiology and Biotechnology}},
  title        = {{Positively Regulated Glycerol/G3P-Dependent <em>Bacillus subtilis</em> Gene Expression System Based on Anti-Termination.}},
  url          = {{http://dx.doi.org/10.1159/000166210}},
  doi          = {{10.1159/000166210}},
  volume       = {{17}},
  year         = {{2009}},
}