Costimulation blockade in transplantation of nerve allografts: long-term effects.
(2008) In Journal of the Peripheral Nervous System 13(3). p.200-207- Abstract
- Costimulation blockade can prevent rejection of nerve allografts in short-term studies. We tested if costimulation blockade also prevented rejection of nerve allografts in long-term experiments, thereby improving functional recovery. A 7-mm sciatic nerve defect in C57/BL6 mice was bridged either by nerve allografts from Balb/C mice or by isogenic nerve grafts (isografts) from C57/BL6 mice. Costimulation blockade in the form of a triple treatment with anti-LFA-1, anti-CD40L, and CTLA4Ig was given at post-operative days 0, 2, 4, and 6 (intraperitoneal). Control mice (placebo; allografts) with nerve grafts were treated with isotype antibodies during the same time period. After 49 days, tetanic muscle force, wet weight of gastrocnemius muscle,... (More)
- Costimulation blockade can prevent rejection of nerve allografts in short-term studies. We tested if costimulation blockade also prevented rejection of nerve allografts in long-term experiments, thereby improving functional recovery. A 7-mm sciatic nerve defect in C57/BL6 mice was bridged either by nerve allografts from Balb/C mice or by isogenic nerve grafts (isografts) from C57/BL6 mice. Costimulation blockade in the form of a triple treatment with anti-LFA-1, anti-CD40L, and CTLA4Ig was given at post-operative days 0, 2, 4, and 6 (intraperitoneal). Control mice (placebo; allografts) with nerve grafts were treated with isotype antibodies during the same time period. After 49 days, tetanic muscle force, wet weight of gastrocnemius muscle, histology, and morphometry in the tibial nerve were evaluated. Costimulation blockade diminished rejection of the nerve allografts. Axons bridged the graft. Treatment increased wet weight of the gastrocnemius muscle and resulted in a higher mean myelin area/nerve fiber in the tibial nerve distal to the nerve grafts. Tetanic muscle force and number of axons in tibial nerve showed no differences between groups. We conclude that rejection is suppressed by costimulation blockade. Treatment improves recovery of target muscle and myelination after nerve allografting. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1262389
- author
- Kvist, Martin LU ; Kanje, Martin LU ; Ekberg, Henrik LU ; Corbascio, Matthias and Dahlin, Lars B
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the Peripheral Nervous System
- volume
- 13
- issue
- 3
- pages
- 200 - 207
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000259613000002
- pmid:18844786
- scopus:52949088299
- pmid:18844786
- ISSN
- 1529-8027
- DOI
- 10.1111/j.1529-8027.2008.00178.x
- project
- Nerve regeneration - signal transduktion mechanisms, timing and alternatives to nerve grafts
- language
- English
- LU publication?
- yes
- id
- 032e99ee-b105-4099-9304-2f8b4b89fef2 (old id 1262389)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18844786?dopt=Abstract
- date added to LUP
- 2016-04-01 13:10:15
- date last changed
- 2022-01-27 17:45:24
@article{032e99ee-b105-4099-9304-2f8b4b89fef2,
abstract = {{Costimulation blockade can prevent rejection of nerve allografts in short-term studies. We tested if costimulation blockade also prevented rejection of nerve allografts in long-term experiments, thereby improving functional recovery. A 7-mm sciatic nerve defect in C57/BL6 mice was bridged either by nerve allografts from Balb/C mice or by isogenic nerve grafts (isografts) from C57/BL6 mice. Costimulation blockade in the form of a triple treatment with anti-LFA-1, anti-CD40L, and CTLA4Ig was given at post-operative days 0, 2, 4, and 6 (intraperitoneal). Control mice (placebo; allografts) with nerve grafts were treated with isotype antibodies during the same time period. After 49 days, tetanic muscle force, wet weight of gastrocnemius muscle, histology, and morphometry in the tibial nerve were evaluated. Costimulation blockade diminished rejection of the nerve allografts. Axons bridged the graft. Treatment increased wet weight of the gastrocnemius muscle and resulted in a higher mean myelin area/nerve fiber in the tibial nerve distal to the nerve grafts. Tetanic muscle force and number of axons in tibial nerve showed no differences between groups. We conclude that rejection is suppressed by costimulation blockade. Treatment improves recovery of target muscle and myelination after nerve allografting.}},
author = {{Kvist, Martin and Kanje, Martin and Ekberg, Henrik and Corbascio, Matthias and Dahlin, Lars B}},
issn = {{1529-8027}},
language = {{eng}},
number = {{3}},
pages = {{200--207}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of the Peripheral Nervous System}},
title = {{Costimulation blockade in transplantation of nerve allografts: long-term effects.}},
url = {{http://dx.doi.org/10.1111/j.1529-8027.2008.00178.x}},
doi = {{10.1111/j.1529-8027.2008.00178.x}},
volume = {{13}},
year = {{2008}},
}