Fragmentation and separation analysis of the photosynthetic membrane from spinach.
(2009) In Biochimica et Biophysica Acta - Bioenergetics 1787(1). p.25-36- Abstract
- Membrane vesicles, originating from grana, grana core (appressed grana regions), grana margins and stroma lamellae/end membranes, were analysed by counter current distribution (CCD) using aqueous dextran-polyethylene glycol two-phase systems. Each vesicle population gave rise to distinct peaks in the CCD diagram representing different vesicle subpopulations. The grana vesicles and grana core vesicles each separated into 3 different subpopulations having different chlorophyll a/b ratios and PSI/PSII ratios. Two of the grana core subpopulations had a chlorophyll a/b ratio of 2.0 and PSI/PSII ratio of 0.10 and are among the most PSII enriched thylakoid vesicle preparation obtained so far by a non detergent method. The margin vesicles... (More)
- Membrane vesicles, originating from grana, grana core (appressed grana regions), grana margins and stroma lamellae/end membranes, were analysed by counter current distribution (CCD) using aqueous dextran-polyethylene glycol two-phase systems. Each vesicle population gave rise to distinct peaks in the CCD diagram representing different vesicle subpopulations. The grana vesicles and grana core vesicles each separated into 3 different subpopulations having different chlorophyll a/b ratios and PSI/PSII ratios. Two of the grana core subpopulations had a chlorophyll a/b ratio of 2.0 and PSI/PSII ratio of 0.10 and are among the most PSII enriched thylakoid vesicle preparation obtained so far by a non detergent method. The margin vesicles separated into 3 different populations, with about the same chlorophyll a/b ratios, but different fluorescence emission spectra. The stroma lamellae/end membrane vesicles separated into 4 subpopulations. Plastoglobules, connected to membrane vesicles, were highly enriched in 2 of these subpopulations and it is proposed that these 2 subpopulations originate from stroma lamellae while the 2 others originate from end membranes. Fragmentation and separation analysis shows that the margins of grana constitute a distinct domain of the thylakoid and also allows the estimation of the chlorophyll antenna sizes of PSI and PSII in different thylakoid domains. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1271279
- author
- Danielsson, Ravi LU and Albertsson, Per-Åke LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochimica et Biophysica Acta - Bioenergetics
- volume
- 1787
- issue
- 1
- pages
- 25 - 36
- publisher
- Elsevier
- external identifiers
-
- wos:000262538800003
- pmid:19022219
- scopus:57749104796
- pmid:19022219
- ISSN
- 0005-2728
- DOI
- 10.1016/j.bbabio.2008.10.002
- language
- English
- LU publication?
- yes
- id
- 7dfe4b83-fa9a-453b-a790-69af1874ebb6 (old id 1271279)
- date added to LUP
- 2016-04-01 14:51:12
- date last changed
- 2022-04-22 05:36:02
@article{7dfe4b83-fa9a-453b-a790-69af1874ebb6,
abstract = {{Membrane vesicles, originating from grana, grana core (appressed grana regions), grana margins and stroma lamellae/end membranes, were analysed by counter current distribution (CCD) using aqueous dextran-polyethylene glycol two-phase systems. Each vesicle population gave rise to distinct peaks in the CCD diagram representing different vesicle subpopulations. The grana vesicles and grana core vesicles each separated into 3 different subpopulations having different chlorophyll a/b ratios and PSI/PSII ratios. Two of the grana core subpopulations had a chlorophyll a/b ratio of 2.0 and PSI/PSII ratio of 0.10 and are among the most PSII enriched thylakoid vesicle preparation obtained so far by a non detergent method. The margin vesicles separated into 3 different populations, with about the same chlorophyll a/b ratios, but different fluorescence emission spectra. The stroma lamellae/end membrane vesicles separated into 4 subpopulations. Plastoglobules, connected to membrane vesicles, were highly enriched in 2 of these subpopulations and it is proposed that these 2 subpopulations originate from stroma lamellae while the 2 others originate from end membranes. Fragmentation and separation analysis shows that the margins of grana constitute a distinct domain of the thylakoid and also allows the estimation of the chlorophyll antenna sizes of PSI and PSII in different thylakoid domains.}},
author = {{Danielsson, Ravi and Albertsson, Per-Åke}},
issn = {{0005-2728}},
language = {{eng}},
number = {{1}},
pages = {{25--36}},
publisher = {{Elsevier}},
series = {{Biochimica et Biophysica Acta - Bioenergetics}},
title = {{Fragmentation and separation analysis of the photosynthetic membrane from spinach.}},
url = {{http://dx.doi.org/10.1016/j.bbabio.2008.10.002}},
doi = {{10.1016/j.bbabio.2008.10.002}},
volume = {{1787}},
year = {{2009}},
}