Nontypeable Haemophilus influenzae Adhesin Protein E: Characterization and Biological Activity.
Ronander, Elena LU ; Brant, Marta LU ; Eriksson, Emily LU ; Mörgelin, Matthias LU ; Hallgren, Oskar LU ; Westergren-Thorsson, Gunilla LU ; Forsgren, Arne LU and Riesbeck, Kristian LU
(2009)
In Journal of Infectious Diseases
199.
p.522-531
- Abstract
- The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on... (More)
- The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on pulmonary challenge than did mice immunized with a control peptide. Finally, anti-PE mouse antibodies from vaccinated mice prevented NTHi adhesion. Our data suggest that the ubiquitous adhesin PE plays an important role in the pathogenesis of NTHi infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1289926
- author
- Ronander, Elena
LU
; Brant, Marta
LU
; Eriksson, Emily
LU
; Mörgelin, Matthias
LU
; Hallgren, Oskar
LU
; Westergren-Thorsson, Gunilla
LU
; Forsgren, Arne
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 199
- pages
- 522 - 531
- publisher
- Oxford University Press
- external identifiers
-
- wos:000262802200008
- pmid:19125675
- scopus:61849119539
- ISSN
- 1537-6613
- DOI
- 10.1086/596211
- language
- English
- LU publication?
- yes
- id
- 1a769c5d-ad5c-40b3-b456-6a41398b817e (old id 1289926)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19125675?dopt=Abstract
- date added to LUP
- 2016-04-04 09:26:39
- date last changed
- 2022-05-17 00:26:18
@article{1a769c5d-ad5c-40b3-b456-6a41398b817e,
abstract = {{The adhesin protein E (PE) of the human respiratory pathogen nontypeable Haemophilus influenzae (NTHi) exists in all clinical isolates. In the present study, NTHi adherence to epithelial cells of various origins was further analyzed. The number of intraepithelial PE-deficient NTHi was decreased compared with PE-expressing NTHi. Interestingly, PE-expressing NTHi or Escherichia coli transformants, in addition to soluble recombinant PE22-160 without a lipid moiety, induced a proinflammatory cell response. The adhesive PE domain was defined within PE84-108, and preincubation of epithelial cells with this peptide blocked adhesion of several clinical NTHi isolates. Mice immunized with PE84-108 cleared NTHi up to 8-fold more efficiently on pulmonary challenge than did mice immunized with a control peptide. Finally, anti-PE mouse antibodies from vaccinated mice prevented NTHi adhesion. Our data suggest that the ubiquitous adhesin PE plays an important role in the pathogenesis of NTHi infection.}},
author = {{Ronander, Elena and Brant, Marta and Eriksson, Emily and Mörgelin, Matthias and Hallgren, Oskar and Westergren-Thorsson, Gunilla and Forsgren, Arne and Riesbeck, Kristian}},
issn = {{1537-6613}},
language = {{eng}},
pages = {{522--531}},
publisher = {{Oxford University Press}},
series = {{Journal of Infectious Diseases}},
title = {{Nontypeable Haemophilus influenzae Adhesin Protein E: Characterization and Biological Activity.}},
url = {{http://dx.doi.org/10.1086/596211}},
doi = {{10.1086/596211}},
volume = {{199}},
year = {{2009}},
}