Integrated immobilized cell reactor–adsorption system for b-cyclodextrin production: a model study using PVA-cryogel entrapped Bacillus agaradhaerens cells.
(2003) In Biotechnology Letters 25(18). p.1537-1543- Abstract
- Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to... (More)
- Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to Amberlite XAD-4 in a recycle batch mode. The product adsorption was facilitated at low temperature while hot water was used for elution. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/129225
- author
- Martins, Rita LU ; Plieva, Fatima LU ; Santos, Ana and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Letters
- volume
- 25
- issue
- 18
- pages
- 1537 - 1543
- publisher
- Springer
- external identifiers
-
- wos:000184957400010
- pmid:14571979
- scopus:0141836934
- ISSN
- 1573-6776
- DOI
- 10.1023/A:1025408727114
- language
- English
- LU publication?
- yes
- id
- 1990dc1f-0a81-4cce-936b-8d42f32e5531 (old id 129225)
- date added to LUP
- 2016-04-01 16:51:13
- date last changed
- 2022-01-28 22:39:22
@article{1990dc1f-0a81-4cce-936b-8d42f32e5531,
abstract = {{Production of cyclodextrins (CDs) by immobilized cells of the alkaliphilic Bacillus agaradhaerens LS-3C with integrated product recovery was studied. The microorganism was entrapped in polyvinyl alcohol-cryogel beads and used as a convenient source of immobilized cyclodextrin glycosyltransferase (CGTase). On activation by incubation in the cultivation medium containing 1% (w/v) starch, the entrapped cells multiplied and secreted CGTase with an activity of 2–3 mg β-cyclodextrin h-1 g-1 beads. The immobilized biocatalyst exhibited maximum activity at pH 9 and 50 °C, and formed cyclodextrins comprising 92–94% β-CD and remaining α-CD. The cyclodextrin product from the immobilized cell bioreactor was continuously recovered by adsorption to Amberlite XAD-4 in a recycle batch mode. The product adsorption was facilitated at low temperature while hot water was used for elution.}},
author = {{Martins, Rita and Plieva, Fatima and Santos, Ana and Hatti-Kaul, Rajni}},
issn = {{1573-6776}},
language = {{eng}},
number = {{18}},
pages = {{1537--1543}},
publisher = {{Springer}},
series = {{Biotechnology Letters}},
title = {{Integrated immobilized cell reactor–adsorption system for b-cyclodextrin production: a model study using PVA-cryogel entrapped Bacillus agaradhaerens cells.}},
url = {{http://dx.doi.org/10.1023/A:1025408727114}},
doi = {{10.1023/A:1025408727114}},
volume = {{25}},
year = {{2003}},
}