Deficiency of oncoretrovirally transduced hematopoietic stem cells and correction through ex vivo expansion.
(2005) In Journal of Gene Medicine 7(2). p.137-144- Abstract
- Background Extensive efforts to develop hematopoietic stem cell (HSC) based gene therapy have been hampered by low gene marking. Major emphasis has so far been directed at improving gene transfer efficiency, but low gene marking in transplanted recipients might equally well reflect compromised repopulating activity of transduced cells, competing for reconstitution with endogenous and unmanipulated stem cells. Methods The autologous settings of clinical gene therapy protocols preclude evaluation of changes in repopulating ability following transduction; however, using a congenic mouse model, allowing for direct evaluation of gene marking of lympho-myeloid progeny, we show here that these issues can be accurately addressed. Results We... (More)
- Background Extensive efforts to develop hematopoietic stem cell (HSC) based gene therapy have been hampered by low gene marking. Major emphasis has so far been directed at improving gene transfer efficiency, but low gene marking in transplanted recipients might equally well reflect compromised repopulating activity of transduced cells, competing for reconstitution with endogenous and unmanipulated stem cells. Methods The autologous settings of clinical gene therapy protocols preclude evaluation of changes in repopulating ability following transduction; however, using a congenic mouse model, allowing for direct evaluation of gene marking of lympho-myeloid progeny, we show here that these issues can be accurately addressed. Results We demonstrate that conditions supporting in vitro stem cell self-renewal efficiently promote oncoretroviral-mediated gene transfer to multipotent adult bone marrow stem cells, without prior in vivo conditioning. Despite using optimized culture conditions, transduction resulted in striking losses of repopulating activity, translating into low numbers of gene marked cells in competitively repopulated mice. Subjecting transduced HSCs to an ex vivo expansion protocol following the transduction procedure could partially reverse this loss. Conclusions These studies suggest that loss of repopulating ability of transduced HSCs rather than low gene transfer efficiency might be the main problem in clinical gene therapy protocols, and that a clinically feasible ex vivo expansion approach post-transduction can markedly improve reconstitution with gene marked stem cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/130881
- author
- Bryder, David LU ; Sasaki, Yutaka LU and Jacobsen, Sten Eirik W LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- transplantation, hematopoiesis, gene therapy
- in
- Journal of Gene Medicine
- volume
- 7
- issue
- 2
- pages
- 137 - 144
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:15538726
- wos:000227253900001
- scopus:14844293836
- ISSN
- 1521-2254
- DOI
- 10.1002/jgm.658
- language
- English
- LU publication?
- yes
- id
- fda2d766-16d3-472e-b4b9-191878ed298d (old id 130881)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15538726&dopt=Abstract
- date added to LUP
- 2016-04-01 11:52:01
- date last changed
- 2022-03-13 01:50:19
@article{fda2d766-16d3-472e-b4b9-191878ed298d,
abstract = {{Background Extensive efforts to develop hematopoietic stem cell (HSC) based gene therapy have been hampered by low gene marking. Major emphasis has so far been directed at improving gene transfer efficiency, but low gene marking in transplanted recipients might equally well reflect compromised repopulating activity of transduced cells, competing for reconstitution with endogenous and unmanipulated stem cells. Methods The autologous settings of clinical gene therapy protocols preclude evaluation of changes in repopulating ability following transduction; however, using a congenic mouse model, allowing for direct evaluation of gene marking of lympho-myeloid progeny, we show here that these issues can be accurately addressed. Results We demonstrate that conditions supporting in vitro stem cell self-renewal efficiently promote oncoretroviral-mediated gene transfer to multipotent adult bone marrow stem cells, without prior in vivo conditioning. Despite using optimized culture conditions, transduction resulted in striking losses of repopulating activity, translating into low numbers of gene marked cells in competitively repopulated mice. Subjecting transduced HSCs to an ex vivo expansion protocol following the transduction procedure could partially reverse this loss. Conclusions These studies suggest that loss of repopulating ability of transduced HSCs rather than low gene transfer efficiency might be the main problem in clinical gene therapy protocols, and that a clinically feasible ex vivo expansion approach post-transduction can markedly improve reconstitution with gene marked stem cells.}},
author = {{Bryder, David and Sasaki, Yutaka and Jacobsen, Sten Eirik W}},
issn = {{1521-2254}},
keywords = {{transplantation; hematopoiesis; gene therapy}},
language = {{eng}},
number = {{2}},
pages = {{137--144}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Gene Medicine}},
title = {{Deficiency of oncoretrovirally transduced hematopoietic stem cells and correction through ex vivo expansion.}},
url = {{http://dx.doi.org/10.1002/jgm.658}},
doi = {{10.1002/jgm.658}},
volume = {{7}},
year = {{2005}},
}