A new nested polymerase chain reaction method very efficient in detecting Plasmodium and Haemoproteus infections from avian blood
(2004) In The Journal of Parasitology 90(1). p.191-194- Abstract
- Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of... (More)
- Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/137056
- author
- Waldenström, Jonas LU ; Bensch, Staffan LU ; Hasselquist, Dennis LU and Östman, Ö
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Parasitology
- volume
- 90
- issue
- 1
- pages
- 191 - 194
- publisher
- American Society of Parasitologists
- external identifiers
-
- wos:000220367300035
- pmid:15040694
- scopus:1442274864
- ISSN
- 0022-3395
- project
- Malaria in birds
- language
- English
- LU publication?
- yes
- id
- 93047ae0-82d2-4675-930e-23c33c635279 (old id 137056)
- alternative location
- http://www.bioone.org/bioone/?request=get-abstract&issn=0022-3395&volume=090&issue=01&page=0191
- date added to LUP
- 2016-04-01 16:37:44
- date last changed
- 2022-04-22 23:23:29
@article{93047ae0-82d2-4675-930e-23c33c635279,
abstract = {{Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods.}},
author = {{Waldenström, Jonas and Bensch, Staffan and Hasselquist, Dennis and Östman, Ö}},
issn = {{0022-3395}},
language = {{eng}},
number = {{1}},
pages = {{191--194}},
publisher = {{American Society of Parasitologists}},
series = {{The Journal of Parasitology}},
title = {{A new nested polymerase chain reaction method very efficient in detecting Plasmodium and Haemoproteus infections from avian blood}},
url = {{http://www.bioone.org/bioone/?request=get-abstract&issn=0022-3395&volume=090&issue=01&page=0191}},
volume = {{90}},
year = {{2004}},
}