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Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus

Söderlund Strand, Anna LU ; Carlson, Joyce LU and Dillner, Joakim LU (2009) In Journal of Clinical Microbiology 47(3). p.541-546
Abstract
Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5 +/6 + using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5 +/6 +) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was... (More)
Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5 +/6 + using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5 +/6 +) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was able to detect all 14 oncogenic HPV types at five copies/PCR. In the clinical samples, the MGP system detected a significantly higher proportion of women with more than two concomitant HPV infections than did the GP5 +/6 + system (102/592 women compared to 42/592 women). MGP detected a significantly greater number of infections with HPV-16, -18, -31, -33, -35, -39, -42, -43, -45, -51, -52, -56, -58, and -70 than did GP5 +/6 +. In summary, the MGP system primers allow a more sensitive amplification of most of the HPV types that are established as oncogenic and had an improved ability to detect multiple concomitant HPV infections. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
47
issue
3
pages
541 - 546
publisher
American Society for Microbiology
external identifiers
  • wos:000263818800004
  • pmid:19144817
  • scopus:62749172924
  • pmid:19144817
ISSN
1098-660X
DOI
10.1128/JCM.02007-08
language
English
LU publication?
yes
id
656017e3-00df-48b7-a2f8-54f84ab0115c (old id 1371121)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19144817?dopt=Abstract
date added to LUP
2016-04-01 11:58:17
date last changed
2022-03-20 21:39:10
@article{656017e3-00df-48b7-a2f8-54f84ab0115c,
  abstract     = {{Human papillomavirus (HPV) infection is a necessary cause of cervical cancer and cervical dysplasia. Accurate and sensitive genotyping of multiple oncogenic HPVs is essential for a multitude of both clinical and research uses. We developed a modified general primer (MGP) PCR system with five forward and five reverse consensus primers. The MGP system was compared to the classical HPV general primer system GP5 +/6 + using a proficiency panel with HPV plasmid dilutions as well as cervical samples from 592 women with low-grade cytological abnormalities. The reference method (GP5 +/6 +) had the desirable high sensitivity (five copies/PCR) for five oncogenic HPV types (HPV type 16 [HPV-16], HPV-18, HPV-56, HPV-59, and HPV-66). The MGP system was able to detect all 14 oncogenic HPV types at five copies/PCR. In the clinical samples, the MGP system detected a significantly higher proportion of women with more than two concomitant HPV infections than did the GP5 +/6 + system (102/592 women compared to 42/592 women). MGP detected a significantly greater number of infections with HPV-16, -18, -31, -33, -35, -39, -42, -43, -45, -51, -52, -56, -58, and -70 than did GP5 +/6 +. In summary, the MGP system primers allow a more sensitive amplification of most of the HPV types that are established as oncogenic and had an improved ability to detect multiple concomitant HPV infections.}},
  author       = {{Söderlund Strand, Anna and Carlson, Joyce and Dillner, Joakim}},
  issn         = {{1098-660X}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{541--546}},
  publisher    = {{American Society for Microbiology}},
  series       = {{Journal of Clinical Microbiology}},
  title        = {{Modified General Primer PCR System for Sensitive Detection of Multiple Types of Oncogenic Human Papillomavirus}},
  url          = {{http://dx.doi.org/10.1128/JCM.02007-08}},
  doi          = {{10.1128/JCM.02007-08}},
  volume       = {{47}},
  year         = {{2009}},
}