Nanospray mass spectrometry with indirect conductive graphite coating
(2004) In Analytical Chemistry 76(14). p.4241-4244- Abstract
- An easy and cost-effective method to manufacture a robust conductive graphite coating for nanospray mass spectrometry (nESI-MS) and capillary electrophoresis (CE)-nESI-MS is described. The method involves graphite coating of a tube sleeve, into which the nESI emitter is inserted and connected to a transfer capillary, instead of coating the actual emitter. The coating, made of graphite from a pencil and epoxy glue, was stable over long periods of use (>80 h) and showed excellent resistance toward various solvents. Stable electrospray was achieved in the investigated flow range (150-900 nL min(-1)), and salbutamol, diphenhydramine, and nortriptyline (M-w: 239-263 g mol(-1)) were detected in the nanomole per liter range during continuous... (More)
- An easy and cost-effective method to manufacture a robust conductive graphite coating for nanospray mass spectrometry (nESI-MS) and capillary electrophoresis (CE)-nESI-MS is described. The method involves graphite coating of a tube sleeve, into which the nESI emitter is inserted and connected to a transfer capillary, instead of coating the actual emitter. The coating, made of graphite from a pencil and epoxy glue, was stable over long periods of use (>80 h) and showed excellent resistance toward various solvents. Stable electrospray was achieved in the investigated flow range (150-900 nL min(-1)), and salbutamol, diphenhydramine, and nortriptyline (M-w: 239-263 g mol(-1)) were detected in the nanomole per liter range during continuous pumping. CE-nESI-MS analysis gave excellent signal-to-noise ratios for 100-fmol injections. The technique allows simple exchange of the nESI emitter to suit a specific flow rate, and it minimizes risk of corona discharge. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138612
- author
- Viberg, Peter LU ; Nilsson, Staffan LU and Skog, Kerstin LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Chemistry
- volume
- 76
- issue
- 14
- pages
- 4241 - 4244
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000222706400052
- pmid:15253670
- scopus:3242676968
- pmid:15253670
- ISSN
- 1520-6882
- DOI
- 10.1021/ac049728e
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Applied Nutrition and Food Chemistry (011001300)
- id
- 89c9ece1-7ea2-4d87-981c-5b00a7ffa238 (old id 138612)
- date added to LUP
- 2016-04-01 12:12:38
- date last changed
- 2023-09-01 23:21:54
@article{89c9ece1-7ea2-4d87-981c-5b00a7ffa238,
abstract = {{An easy and cost-effective method to manufacture a robust conductive graphite coating for nanospray mass spectrometry (nESI-MS) and capillary electrophoresis (CE)-nESI-MS is described. The method involves graphite coating of a tube sleeve, into which the nESI emitter is inserted and connected to a transfer capillary, instead of coating the actual emitter. The coating, made of graphite from a pencil and epoxy glue, was stable over long periods of use (>80 h) and showed excellent resistance toward various solvents. Stable electrospray was achieved in the investigated flow range (150-900 nL min(-1)), and salbutamol, diphenhydramine, and nortriptyline (M-w: 239-263 g mol(-1)) were detected in the nanomole per liter range during continuous pumping. CE-nESI-MS analysis gave excellent signal-to-noise ratios for 100-fmol injections. The technique allows simple exchange of the nESI emitter to suit a specific flow rate, and it minimizes risk of corona discharge.}},
author = {{Viberg, Peter and Nilsson, Staffan and Skog, Kerstin}},
issn = {{1520-6882}},
language = {{eng}},
number = {{14}},
pages = {{4241--4244}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Analytical Chemistry}},
title = {{Nanospray mass spectrometry with indirect conductive graphite coating}},
url = {{http://dx.doi.org/10.1021/ac049728e}},
doi = {{10.1021/ac049728e}},
volume = {{76}},
year = {{2004}},
}