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Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale

Rudolf, Andreas LU ; Galbe, Mats LU and Lidén, Gunnar LU (2004) In Applied Biochemistry and Biotechnology 114(1-3). p.601-617
Abstract
Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the... (More)
Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
114
issue
1-3
pages
601 - 617
publisher
Humana Press
external identifiers
  • wos:000221186200050
  • scopus:11244315135
ISSN
1559-0291
DOI
10.1385/ABAB:114:1-3:601
language
English
LU publication?
yes
id
51d38725-f2f9-4c60-ae40-ed953f6d56dd (old id 138861)
date added to LUP
2016-04-01 11:33:30
date last changed
2023-11-10 16:43:46
@article{51d38725-f2f9-4c60-ae40-ed953f6d56dd,
  abstract     = {{Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted.}},
  author       = {{Rudolf, Andreas and Galbe, Mats and Lidén, Gunnar}},
  issn         = {{1559-0291}},
  language     = {{eng}},
  number       = {{1-3}},
  pages        = {{601--617}},
  publisher    = {{Humana Press}},
  series       = {{Applied Biochemistry and Biotechnology}},
  title        = {{Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale}},
  url          = {{http://dx.doi.org/10.1385/ABAB:114:1-3:601}},
  doi          = {{10.1385/ABAB:114:1-3:601}},
  volume       = {{114}},
  year         = {{2004}},
}