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Novel peptide surface for reversible immobilization of concanavalin A

Masarova, Jana LU ; Dey, Estera LU ; Carlsson, J and Danielsson, Bengt LU (2004) In Journal of Biochemical and Biophysical Methods 60(2). p.163-170
Abstract
Concanavalin A (Con A) was spontaneously adsorbed on polymyxin B surface. This peptide-lectin interaction was strong, K-D = 1-9 X 10(-10), based predominantly on creation of hydrophobic bonds, and was completely reversible. Concanavalin A on polymyxin B (PmB) retained higher binding capacity for yeast mannan, compared with covalently immobilized lectin. Kinetics of mannan-concanavalin A interaction were significantly different in dependence on type of concanavalin A immobilization. (C) 2004 Elsevier B.V All rights reserved.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Immobilization, Hydrophobic interaction, Concanavalin A, Polymyxin B, Surface plasmon resonance
in
Journal of Biochemical and Biophysical Methods
volume
60
issue
2
pages
163 - 170
publisher
Elsevier
external identifiers
  • wos:000223463400006
  • scopus:3242713049
ISSN
0165-022X
DOI
10.1016/j.jbbm.2004.05.005
language
English
LU publication?
yes
id
e7e463ee-0044-4384-8c2a-2d53f86d3b46 (old id 140998)
date added to LUP
2016-04-01 15:55:01
date last changed
2022-01-28 08:00:36
@article{e7e463ee-0044-4384-8c2a-2d53f86d3b46,
  abstract     = {{Concanavalin A (Con A) was spontaneously adsorbed on polymyxin B surface. This peptide-lectin interaction was strong, K-D = 1-9 X 10(-10), based predominantly on creation of hydrophobic bonds, and was completely reversible. Concanavalin A on polymyxin B (PmB) retained higher binding capacity for yeast mannan, compared with covalently immobilized lectin. Kinetics of mannan-concanavalin A interaction were significantly different in dependence on type of concanavalin A immobilization. (C) 2004 Elsevier B.V All rights reserved.}},
  author       = {{Masarova, Jana and Dey, Estera and Carlsson, J and Danielsson, Bengt}},
  issn         = {{0165-022X}},
  keywords     = {{Immobilization; Hydrophobic interaction; Concanavalin A; Polymyxin B; Surface plasmon resonance}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{163--170}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Biochemical and Biophysical Methods}},
  title        = {{Novel peptide surface for reversible immobilization of concanavalin A}},
  url          = {{http://dx.doi.org/10.1016/j.jbbm.2004.05.005}},
  doi          = {{10.1016/j.jbbm.2004.05.005}},
  volume       = {{60}},
  year         = {{2004}},
}