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A modified ELISA for improved detection of IgA, IgG, and IgM anti-tissue transglutaminase antibodies in celiac disease

Teesalu, Kaupo ; Agardh, Daniel LU ; Panarina, Marina ; Utt, Meeme LU ; Uibo, Oivi and Uibo, Raivo LU (2009) In Clinica Chimica Acta 403(1-2). p.37-41
Abstract
Background: Serum IgA antibodies against tissue transglutaminase (tTG) are reliable markers for celiac disease (CD), still the diagnostic performance of anti-tTG immunoassays can be improved. A novel ELISA, using fibronectin (FN) as tTG binding protein, was evaluated for the detection of anti-tTG antibodies in CD. Methods: Sera from 173 children with untreated CD and 97 controls were analyzed for IgA, IgG, and IgM anti-tTG antibodies with ELISA using human recombinant tTG with or without FN. Results: The areas under the ROC (receiver operating characteristic) curves were significantly higher for FN/tTG ELISA compared to tTG ELISA for IgG (0.930 versus 0.809; p < 0.001) and IgM (0.850 versus 0.811; p = 0.019), but not for IgA (0.977... (More)
Background: Serum IgA antibodies against tissue transglutaminase (tTG) are reliable markers for celiac disease (CD), still the diagnostic performance of anti-tTG immunoassays can be improved. A novel ELISA, using fibronectin (FN) as tTG binding protein, was evaluated for the detection of anti-tTG antibodies in CD. Methods: Sera from 173 children with untreated CD and 97 controls were analyzed for IgA, IgG, and IgM anti-tTG antibodies with ELISA using human recombinant tTG with or without FN. Results: The areas under the ROC (receiver operating characteristic) curves were significantly higher for FN/tTG ELISA compared to tTG ELISA for IgG (0.930 versus 0.809; p < 0.001) and IgM (0.850 versus 0.811; p = 0.019), but not for IgA (0.977 versus 0.970; p = 0.356), respectively. At the fixed diagnostic specificity (100% for IgA and IgM, 99% for IgG), the sensitivity of all three FN/tTG ELISA (92.5% for IgA, 60.7% for IgG, 50.3% for IgM) exceeded those obtained in tTG ELISA (89.0% for IgA, 48.6% for IgG, 38.7% for IgM; p < 0.05). The combined use of IgA- and IgG-FN/tTG ELISA resulted in 95.4% sensitivity and 99.0% specificity for CD. Conclusions: Using FN to bind tTG improves the diagnostic performance of solid phase anti-tTG antibody assays for childhood CD. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fibronectin, Anti-tissue transglutaminase antibodies, ELISA, disease, Celiac
in
Clinica Chimica Acta
volume
403
issue
1-2
pages
37 - 41
publisher
Elsevier
external identifiers
  • wos:000266190100006
  • scopus:64849087024
ISSN
0009-8981
DOI
10.1016/j.cca.2009.01.006
language
English
LU publication?
yes
id
1929a7d2-3c77-4646-b8e1-6edbbcd35afe (old id 1425699)
date added to LUP
2016-04-01 11:39:28
date last changed
2022-04-28 18:06:08
@article{1929a7d2-3c77-4646-b8e1-6edbbcd35afe,
  abstract     = {{Background: Serum IgA antibodies against tissue transglutaminase (tTG) are reliable markers for celiac disease (CD), still the diagnostic performance of anti-tTG immunoassays can be improved. A novel ELISA, using fibronectin (FN) as tTG binding protein, was evaluated for the detection of anti-tTG antibodies in CD. Methods: Sera from 173 children with untreated CD and 97 controls were analyzed for IgA, IgG, and IgM anti-tTG antibodies with ELISA using human recombinant tTG with or without FN. Results: The areas under the ROC (receiver operating characteristic) curves were significantly higher for FN/tTG ELISA compared to tTG ELISA for IgG (0.930 versus 0.809; p &lt; 0.001) and IgM (0.850 versus 0.811; p = 0.019), but not for IgA (0.977 versus 0.970; p = 0.356), respectively. At the fixed diagnostic specificity (100% for IgA and IgM, 99% for IgG), the sensitivity of all three FN/tTG ELISA (92.5% for IgA, 60.7% for IgG, 50.3% for IgM) exceeded those obtained in tTG ELISA (89.0% for IgA, 48.6% for IgG, 38.7% for IgM; p &lt; 0.05). The combined use of IgA- and IgG-FN/tTG ELISA resulted in 95.4% sensitivity and 99.0% specificity for CD. Conclusions: Using FN to bind tTG improves the diagnostic performance of solid phase anti-tTG antibody assays for childhood CD. (C) 2009 Elsevier B.V. All rights reserved.}},
  author       = {{Teesalu, Kaupo and Agardh, Daniel and Panarina, Marina and Utt, Meeme and Uibo, Oivi and Uibo, Raivo}},
  issn         = {{0009-8981}},
  keywords     = {{Fibronectin; Anti-tissue transglutaminase antibodies; ELISA; disease; Celiac}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{37--41}},
  publisher    = {{Elsevier}},
  series       = {{Clinica Chimica Acta}},
  title        = {{A modified ELISA for improved detection of IgA, IgG, and IgM anti-tissue transglutaminase antibodies in celiac disease}},
  url          = {{http://dx.doi.org/10.1016/j.cca.2009.01.006}},
  doi          = {{10.1016/j.cca.2009.01.006}},
  volume       = {{403}},
  year         = {{2009}},
}