Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries
(2009) In BMC Biotechnology 9.- Abstract
- Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional... (More)
- Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure resulting in 4-8 fold decrease of total time needed for phage selection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1428033
- author
- Noppe, Wim LU ; Plieva, Fatima LU ; Galaev, Igor LU ; Pottel, Hans ; Deckmyn, Hans and Mattiasson, Bo LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BMC Biotechnology
- volume
- 9
- publisher
- BioMed Central (BMC)
- external identifiers
-
- wos:000265608000001
- scopus:64849104120
- pmid:19292898
- ISSN
- 1472-6750
- DOI
- 10.1186/1472-6750-9-21
- language
- English
- LU publication?
- yes
- id
- c02feb63-0c75-498d-9b63-6043d6df0ab2 (old id 1428033)
- date added to LUP
- 2016-04-01 13:48:57
- date last changed
- 2022-03-14 02:05:07
@article{c02feb63-0c75-498d-9b63-6043d6df0ab2,
abstract = {{Background: Phage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library. Results: Both phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages. Conclusion: Chromato-panning allows combining several steps of the panning procedure resulting in 4-8 fold decrease of total time needed for phage selection.}},
author = {{Noppe, Wim and Plieva, Fatima and Galaev, Igor and Pottel, Hans and Deckmyn, Hans and Mattiasson, Bo}},
issn = {{1472-6750}},
language = {{eng}},
publisher = {{BioMed Central (BMC)}},
series = {{BMC Biotechnology}},
title = {{Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries}},
url = {{http://dx.doi.org/10.1186/1472-6750-9-21}},
doi = {{10.1186/1472-6750-9-21}},
volume = {{9}},
year = {{2009}},
}